Generation of Induced Pluripotent Stem Cells from Human Cord Blood

被引:370
作者
Haase, Alexandra [1 ]
Olmer, Ruth [1 ]
Schwanke, Kristin [1 ]
Wunderlich, Stephanie [1 ]
Merkert, Sylvia [1 ]
Hess, Christian [1 ]
Zweigerdt, Robert [1 ]
Gruh, Ina [1 ]
Meyer, Johann [2 ]
Wagner, Stefan [7 ]
Maier, Lars S. [7 ]
Han, Dong Wook [6 ]
Glage, Silke [3 ]
Miller, Konstantin [4 ]
Fischer, Philipp [5 ]
Schoeler, Hans R. [6 ]
Martin, Ulrich [1 ]
机构
[1] Hannover Med Sch, Dept Cardiac Thorac Transplantat & Vasc Surg, Leibniz Res Labs Biotechnol & Artificial Organs, D-30625 Hannover, Germany
[2] Hannover Med Sch, Dept Expt Hematol, D-30625 Hannover, Germany
[3] Hannover Med Sch, Inst Lab Anim Sci, D-30625 Hannover, Germany
[4] Hannover Med Sch, Dept Human Genet, D-30625 Hannover, Germany
[5] Hannover Med Sch, Dept Cardiol & Angiol, D-30625 Hannover, Germany
[6] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany
[7] Univ Gottingen, Dept Cardiol & Pneumol, D-37075 Gottingen, Germany
关键词
STEMCELL;
D O I
10.1016/j.stem.2009.08.021
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Induced pluripotent stem cells (iPSCs) may represent an ideal cell source for future regenerative therapies. A critical issue concerning the clinical use of patient-specific iPSCs is the accumulation of mutations in somatic (stem) cells over an organism's lifetime. Acquired somatic mutations are passed onto iPSCs during reprogramming and may be associated with loss of cellular functions and cancer formation. Here we report the generation of human iPSCs from cord blood (CB) as a juvenescent cell source. CBiPSCs show characteristics typical of embryonic stem cells and can be differentiated into derivatives of all three germ layers, including functional cardiomyocytes. For future therapeutic production of autologous and allogeneic iPSC derivatives, CB could be routinely harvested for public and commercial CB banks without any donor risk. CB could readily become available for pediatric patients and, in particular, for newborns with genetic diseases or congenital malformations.
引用
收藏
页码:434 / 441
页数:8
相关论文
共 14 条
  • [1] Mobilizing stem cells from normal donors: is it possible to improve upon G-CSF?
    Cashen, A. F.
    Lazarus, H. M.
    Devine, S. M.
    [J]. BONE MARROW TRANSPLANTATION, 2007, 39 (10) : 577 - 588
  • [2] Generation of functional cardiomyocytes from adult mouse spermatogonial stem cells
    Guan, Kaomei
    Wagner, Stefan
    Unsoeld, Bernhard
    Maier, Lars S.
    Kaiser, Diana
    Hemmerlein, Bernhard
    Nayernia, Karim
    Engel, Wolfgang
    Hasenfuss, Gerd
    [J]. CIRCULATION RESEARCH, 2007, 100 (11) : 1615 - 1625
  • [3] Ex vivo expansion of umbilical cord blood stem cells for transplantation:: growing knowledge from the hematopoietic niche
    Hofmeister, C. C.
    Zhang, J.
    Knight, K. L.
    Le, P.
    Stiff, P. J.
    [J]. BONE MARROW TRANSPLANTATION, 2007, 39 (01) : 11 - 23
  • [4] Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood
    Ingram, DA
    Mead, LE
    Tanaka, H
    Meade, V
    Fenoglio, A
    Mortell, K
    Pollok, K
    Ferkowicz, MJ
    Gilley, D
    Yoder, MC
    [J]. BLOOD, 2004, 104 (09) : 2752 - 2760
  • [5] Generation of induced pluripotent stem cells from human blood
    Loh, Yuin-Han
    Agarwal, Suneet
    Park, In-Hyun
    Urbach, Achia
    Huo, Hongguang
    Heffner, Garrett C.
    Kim, Kitai
    Miller, Justine D.
    Ng, Kitwa
    Daley, George Q.
    [J]. BLOOD, 2009, 113 (22) : 5476 - 5479
  • [6] A high-efficiency system for the generation and study of human induced pluripotent stem cells
    Maherali, Nimet
    Ahfeldt, Tim
    Rigamonti, Alessandra
    Utikal, Jochen
    Cowan, Chad
    Hochedlinger, Konrad
    [J]. CELL STEM CELL, 2008, 3 (03) : 340 - 345
  • [7] Telomeres Acquire Embryonic Stem Cell Characteristics in Induced Pluripotent Stem Cells
    Marion, Rosa M.
    Strati, Katerina
    Li, Han
    Tejera, Agueda
    Schoeftner, Stefan
    Ortega, Sagrario
    Serrano, Manuel
    Blasco, Maria A.
    [J]. CELL STEM CELL, 2009, 4 (02) : 141 - 154
  • [8] Generation of functional murine cardiac myocytes from induced pluripotent stem cells
    Mauritz, Christina
    Schwanke, Kristin
    Reppel, Michael
    Neef, Stefan
    Katsirntaki, Katherina
    Maier, Lars S.
    Nguemo, Filomain
    Menke, Sandra
    Haustein, Moritz
    Hescheler, Juergen
    Hasenfuss, Gerd
    Martin, Ulrich
    [J]. CIRCULATION, 2008, 118 (05) : 507 - 517
  • [9] Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization
    Murohara, T
    Ikeda, H
    Duan, J
    Shintani, S
    Sasaki, K
    Eguchi, H
    Onitsuka, I
    Matsui, K
    Imaizumi, T
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2000, 105 (11) : 1527 - 1536
  • [10] Mutation theory of aging, assessed in transgenic mice and knockout mice
    Ono, T
    Uehara, Y
    Saito, Y
    Ikehata, H
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 2002, 123 (12) : 1543 - 1552