Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and workers in China

被引：152

作者：

Cui, Shenghui ^{[1
]}

Li, Jingyun ^{[1
]}

Hu, Changqin ^{[1
]}

Jin, Shaohong ^{[1
]}

Li, Fengqin ^{[2
]}

Guo, Yunchang ^{[2
]}

Ran, Lu ^{[2
]}

Ma, Yue ^{[1
]}

机构：

[1] State Food & Drug Adm, Beijing, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, Beijing, Peoples R China

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2009年 / 64卷 / 04期

关键词：

antimicrobial susceptibility testing; multilocus sequence typing; pulsed field gel electrophoresis; FIELD GEL-ELECTROPHORESIS; PIGS;

D O I：

10.1093/jac/dkp275

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The objectives of this study were to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in livestock and related workers in four Chinese provinces and the characteristics of these isolates. Methods: Nasal swabs were collected from animals and farm workers in four Chinese provinces. MRSA isolates were recovered and characterized by PFGE, Panton-Valentine leucocidin PCR, staphylococcal chromosomal cassette (SCC) mec typing, spa typing, multilocus sequence typing, antimicrobial susceptibility testing and testing for inducible clindamycin resistance. Results: A total of 60 MRSA isolates were recovered from swine and swine workers. Two predominant multidrug resistance profiles were identified: ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline/chloramphenicol and ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline. All isolates were determined to be spa type t899, contained the group III SCCmec element and were Panton-Valentine leucocidin negative. Multilocus sequence type ST9 (n = 46) was identified as the dominant sequence type. One dominant PFGE cluster and a dominant strain type were identified. Conclusions: MRSA from Chinese pigs and farm workers (ST9) differed from the European pig-associated clone (ST398) with regard to clonal type, SCCmec content and resistance profile.

引用

页码：680 / 683

页数：4

共 10 条

[1] Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs [J].