Rapid identification of rough Brucella isolates by a latex coagglutination assay with the 25-kilodalton outer membrane protein and rough-lipopolysaccharide-specific monoclonal antibodies

被引：8

作者：

Bowden, RA ^{[1
]}

Verger, JM ^{[1
]}

Grayon, M ^{[1
]}

Cloeckaert, A ^{[1
]}

机构：

[1] INRA,PATHOL INFECT & IMMUNOL LAB,CTR RECH TOURS,F-37380 NOUZILLY,FRANCE

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1997年 / 4卷 / 05期

关键词：

D O I：

10.1128/CDLI.4.5.611-614.1997

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A latex coagglutination assay was developed to identify rough (R) isolates of Brucella. Latex beads were coated, via protein A, with either an anti-Brucella rough-lipopolysaccharide (R-LPS) monoclonal antibody (MAb) or an anti-Brucella 25-kDa outer membrane protein (Omp25) MAb, Slide agglutination tests were done for 68 strains of Brucella spp., including type strains of all biovars as well as field isolates, Latex treads coated with MAb to R-LPS coagglutinated only R strains, whereas latex beads coated with MAb to Omp25 coagglutinated all the R Brucella isolates except Brucella ovis. Coagglutination was easier to read than agglutination with rabbit R-Brucella-specific antiserum. Thus, this assay accurately differentiates B. ovis from other R Brucella isolates, The latex coagglutination assay can substitute, to advantage, for the current anti-Brucella (R) rabbit monospecific serum.

引用

页码：611 / 614

页数：4

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