A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors

被引：9

作者：

Nishikimi, A

Mukai, J

Kioka, N

Yamada, M ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Reprod Physiol Lab, Kyoto 6068502, Japan

[2] Kyoto Univ, Grad Sch Agr, Biochem Lab, Kyoto 6068502, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1999年 / 1435卷 / 1-2期

基金：

日本学术振兴会;

关键词：

pre-mRNA splicing factor; Prp1p/Zer1p; anti-peptide antibody; tetratrico peptide repeat; leucine zipper;

D O I：

10.1016/S0167-4838(99)00203-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a novel 100-kDa mammalian protein, which was recognized by an anti-peptide antibody against an epitope-containing nuclear localization signal of NF-kappa B p65 subunit. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：147 / 152

页数：6

共 10 条

[1] THE YEAST PRP6 GENE ENCODES A U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE (SNRNP) PROTEIN, AND THE PRP9 GENE ENCODES A PROTEIN REQUIRED FOR U2 SNRNP BINDING [J].