Characteristic patterns of N Oct-3 binding to a set of neuronal promoters

被引:14
作者
Blaud, M [1 ]
Vossen, C [1 ]
Joseph, G [1 ]
Alazard, R [1 ]
Erard, M [1 ]
Nieto, L [1 ]
机构
[1] CNRS, Lab Pharmacol & Biol Struct, F-31077 Toulouse, France
关键词
POU; neuronal promoters; N Oct-3; HNF-3; beta;
D O I
10.1016/j.jmb.2004.04.035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
N Oct-3, a neurospecific POU protein, homodimerizes in a non-cooperative fashion on the neuronal aromatic L-amino acid decarboxylase gene promoter and generates heterodimers with HNF-3beta. Several other neuronal gene promoters, the corticotropin releasing hormone and the aldolase C gene promoters also contain overlapping binding sites for N Oct-3 and HNF-3beta. We have demonstrated that N Oct-3 presents a non-cooperative homo-dimerization on these two additional targets and can also give rise to heterodimers with HNF-3beta. Surprisingly, despite the high degree of conservation of the respective POU subunits, the ubiquitous POU protein Oct-1 can only form monomers even in the presence of either N Oct-3 or HNF-3beta on these DNA targets. Our data indicate that this difference is correlated with the specific ability of a portion of the N Oct-3 linker to fold as an a-helix, a property shared by class III POU proteins. These results suggest that this novel binding pattern permits the hetero-dimerization of N Oct-3 and HNF-3beta on the neuronal promoters, which could be a key issue in the development of the nervous system and possibly tumors of neural origin. (C) 2004 Elsevier Ltd. All rights reserved.
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页码:1049 / 1058
页数:10
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