RA-VII, a cyclic depsipeptide, changes the conformational structure of actin to cause G2 arrest by the inhibition of cytokinesis

被引：40

作者：

Fujiwara, H

Saito, S

Hitotsuyanagi, Y

Takeya, K

Ohizumi, Y ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Pharmaceut Mol Biol, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tokyo Univ Pharm & Life Sci, Sch Pharm, Hachioji, Tokyo 1920392, Japan

来源：

CANCER LETTERS | 2004年 / 209卷 / 02期

关键词：

RA-VII; G(2) arrest; binucleation; F-actin;

D O I：

10.1016/j.canlet.2003.12.022

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In L 12 10 cells, RA-VII (0.1-100 nM) caused the concentration-dependent inhibition of the proliferation and G(2) arrest. Treatment of PCl2 cells with 10 nM RA-VII changed cell shape round with binucleation, suggesting the inhibition of cytokinesis. The fluorescence intensity of FITC-phalloidin bound to F-actin was enhanced by RA-VII. In surface plasmon resonance experiments, the signal of F-actin was modified by RA-VII in close agreement with a concentration of FITC-phalloidin binding to F-actin. These results suggest that RA-VII causes the conformational change of F-actin and the stabilization of actin filaments to induce G(2) arrest. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：223 / 229

页数：7

共 25 条

[1] (-)-doliculide, a new macrocyclic depsipeptide enhancer of actin assembly [J].