Humoral and cellular immune responses following vaccination with purified recombinant hemagglutinin from influenza A (H3N2) virus

被引:37
作者
Powers, DC
McElhaney, JE
Florendo, OA
Manning, MC
Upshaw, CM
Bentley, DW
Wilkinson, BE
机构
[1] VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN, ST LOUIS, MO 63125 USA
[2] ST LOUIS UNIV, VACCINE TREATMENT & EVALUAT UNIT, ST LOUIS, MO 63103 USA
[3] ST LOUIS UNIV, DEPT INTERNAL MED, ST LOUIS, MO 63103 USA
[4] UNIV ALBERTA, DEPT INTERNAL MED, DIV GERIATR MED, EDMONTON, AB, CANADA
[5] MICROGENESYS INC, MERIDEN, CT USA
关键词
D O I
10.1093/infdis/175.2.342
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Adults were immunized with either baculovirus-expressed, purified recombinant hemagglutinin (rHA) from influenza A/Beijing/32/92 (H3N2) virus or saline placebo and evaluated for humoral and in vitro cellular immune responses, Compared with responses in placebo recipients, vaccinees had greater postvaccination H3(Beijing/32) HA (H3)-Specific lymphoproliferation and interleukin (IL)-2, IL-10, and interferon-gamma (LFN-gamma) production, Mean increases in the production of IL-10 (greater than or equal to 20-fold) and IL-2 (10-fold) were relatively greater than that of IFN-gamma (4-fold) or IL-4 (no change), Serum H3 antibodies were induced in 80% of rHA recipients, and the rise in antibody titer was significantly correlated with changes in IL-2, IL-10, and IFN-gamma concentrations, Vaccination with rHA only minimally enhanced anti-influenza virus cytotoxic T lymphocyte activity, These data demonstrate that rHA immunization of adults elicits a significant recall response by memory B and T lymphocytes and suggest that the cytokine response to vaccination has a T helper cell type 0-like profile.
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页码:342 / 351
页数:10
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