Antibiotic resistance gene cassettes derived from the Omega interposon for use in E-coli and Streptomyces

被引：182

作者：

BlondeletRouault, MH

Weiser, J

Lebrihi, A

Branny, P

Pernodet, JL

机构：

[1] UNIV PARIS 11,INST GENET & MICROBIOL,CNRS,URA 2225,F-91405 ORSAY,FRANCE

[2] ACAD SCI CZECH REPUBL,INST MICROBIOL,CS-14200 PRAGUE 4,CZECH REPUBLIC

[3] INST NATL POLYTECH LORRAINE,ENSAIA,LAB MICROBIOL IND,F-54500 VANDOEUVRE NANCY,FRANCE

来源：

GENE | 1997年 / 190卷 / 02期

关键词：

aminoglycoside acetyltransferase; hygromycin phosphotransferase; viomycin phosphotransferase;

D O I：

10.1016/S0378-1119(97)00014-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Three antibiotic resistance gene cassettes, derived from the SZ interposon (Prentki and Krisch (1984) Gene 29, 303-313) were constructed, These cassettes carry different antibiotic resistance genes, conferring resistance to geneticin, hygromycin or viomycin, flanked by short inverted repeats containing transcription and translation termination signals and synthetic polylinkers. These cassettes were designated Omega aac, Omega hyg and Omega vph. Resistance phenotypes conferred by these constructions are selectable in E. coli and Streptomyces. These cassettes can be used for insertional mutagenesis or for vector construction. (C) Elsevier Science B.V.

引用

页码：315 / 317

页数：3

共 12 条

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