Sucrose-phosphate synthase activity and yield analysis of tomato plants transformed with maize sucrose-phosphate synthase

被引:56
作者
Laporte, MM
Galagan, JA
Shapiro, JA
Boersig, MR
Shewmaker, CK
Sharkey, TD
机构
[1] CALGENE INC,DAVIS,CA 95616
[2] BHN RES,BONITA SPRINGS,FL 34135
关键词
cauliflower mosaic virus 35S promoter; growth chamber (open topped) Lycopersicon; open top chambers; ribulose-1,5-bisphosphate carboxylase-oxygenase small subunit promoter; sucrose-phosphate synthase; yield (transformed tomato);
D O I
10.1007/s004250050189
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Sucrose synthesis is a major element of the interactions between photosynthesis and plant growth and development. Tomato (Lycopersicon esculentum Mill. cv. UC82B) plants transformed with maize sucrose-phosphate synthase (SPS; EC 2.3.1.14) expressed from either a ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) small subunit promoter (SSU) or the cauliflower mosaic virus 35S promoter (35S) were used to study effects of increased sucrose synthesis rates on plant growth. The plants were grown in growth chambers, field plots, and open-top chambers. The 35S plants had a 2 to 3-fold increase in young-leaf SPS activity, a 10 to 20-fold increase in young-root SPS activity and no increase in young-fruit SPS activity. The leaf SPS activity in;one of the 35S lines fell to control levels by two months of age. The SSU plants had a 4 to 5-fold increase in leaf SPS activity and no significant increase in root or young-fruit SPS activity. One 35S line, which maintained high leaf SPS activity throughout development, yielded 70-80% more than controls at both normal and elevated CO2 in open-top chambers in the field and 20-30% more than controls in two additional field trials. The other 35S line and the two SSU lines either yielded less or did not differ from controls under several growth conditions. Since only one of four transformed lines showed an increase in yield, we can not yet conclude that increased leaf SPS activity leads to increased yield. However, increased leaf SPS activity appears to result in increased fruit sugar content since all three lines with increased leaf SPS usually also had increased fruit sugars.
引用
收藏
页码:253 / 259
页数:7
相关论文
共 20 条
[1]   A meta-analysis of leaf gas exchange and nitrogen in trees grown under elevated carbon dioxide [J].
Curtis, PS .
PLANT CELL AND ENVIRONMENT, 1996, 19 (02) :127-137
[2]   EFFECTS OF ELEVATED SUCROSE-PHOSPHATE SYNTHASE ACTIVITY ON PHOTOSYNTHESIS, ASSIMILATE PARTITIONING, AND GROWTH IN TOMATO (LYCOPERSICON-ESCULENTUM VAR UC82B) [J].
GALTIER, N ;
FOYER, CH ;
HUBER, J ;
VOELKER, TA ;
HUBER, SC .
PLANT PHYSIOLOGY, 1993, 101 (02) :535-543
[3]   EFFECTS OF LIGHT AND ATMOSPHERIC CARBON-DIOXIDE ENRICHMENT ON PHOTOSYNTHESIS AND CARBON PARTITIONING IN THE LEAVES OF TOMATO (LYCOPERSICON-ESCULENTUM L) PLANTS OVER-EXPRESSING SUCROSE-PHOSPHATE SYNTHASE [J].
GALTIER, N ;
FOYER, CH ;
MURCHIE, E ;
ALRED, R ;
QUICK, P ;
VOELKER, TA ;
THEPENIER, C ;
LASCEVE, G ;
BETSCHE, T .
JOURNAL OF EXPERIMENTAL BOTANY, 1995, 46 :1335-1344
[4]   EFFECTS OF TRANSLOCATION AND ASSIMILATE DEMAND ON PHOTOSYNTHESIS [J].
GEIGER, DR .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1976, 54 (20) :2337-2345
[5]   Role and regulation of sucrose-phosphate synthase in higher plants [J].
Huber, SC ;
Huber, JL .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1996, 47 :431-444
[6]   Carbohydrate-modulated gene expression in plants [J].
Koch, KE .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1996, 47 :509-540
[7]   REGULATION OF THE EXPRESSION OF RBCS AND OTHER PHOTOSYNTHETIC GENES BY CARBOHYDRATES - A MECHANISM FOR THE SINK REGULATION OF PHOTOSYNTHESIS [J].
KRAPP, A ;
HOFMANN, B ;
SCHAFER, C ;
STITT, M .
PLANT JOURNAL, 1993, 3 (06) :817-828
[8]   LIMITATION OF PHOTOSYNTHESIS BY CHANGES IN TEMPERATURE - FACTORS AFFECTING THE RESPONSE OF CARBON-DIOXIDE ASSIMILATION TO TEMPERATURE IN BARLEY LEAVES [J].
LABATE, CA ;
LEEGOOD, RC .
PLANTA, 1988, 173 (04) :519-527
[9]  
MICALLEF BJ, 1995, PLANTA, V196, P327, DOI 10.1007/BF00201392
[10]   Responses to elevated CO2 of Flaveria linearis plants having a reduced activity of cytosolic fructose-1,6-bisphosphatase [J].
Micallef, BJ ;
Vanderveer, PJ ;
Sharkey, TD .
PLANT CELL AND ENVIRONMENT, 1996, 19 (01) :10-16