Immunohistochemical localization of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in rat tissues

被引:101
作者
Kotera, J [1 ]
Fujishige, K [1 ]
Omori, K [1 ]
机构
[1] Tanabe Seiyaku Co Ltd, Discovery Res Lab, Toda, Saitama 3358505, Japan
关键词
cGMP; phosphodiesterase; cerebellum; kidney; pancreas;
D O I
10.1177/002215540004800512
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We raised a polyclonal antibody against maltose binding protein fusion human cGMP-binding, cGMP-specific phosphodiesterase (PDE5) produced in E. coli. This antibody immunoreacted specifically with recombinant human and rat PDE5 proteins expressed in transfected COS-7 cells and with a native form of PDE5 in extracts of rat platelets, lung, and cerebellum. Immunohistochemical analysis showed that the anti-PDE5 antibody detected immunoactive materials in Purkinje cell layers of the cerebellum, proximal renal tubules, collecting renal ducts, and epithelial cells of pancreatic ducts in rats. Reverse transcriptase-polymerase chain reaction analysis demonstrated that PDE5 transcripts are also present in rat cerebellum, kidney, and pancreas. Here we described a cell-specific localization of PDE5 in various rat tissues, suggesting the possibility of the presence of a cGMP/PDE5 pathway in these tissues.
引用
收藏
页码:685 / 693
页数:9
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