Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus

被引：151

作者：

Goto, K ^{[1
]}

Omura, T

Hara, Y

Sadaie, Y

机构：

[1] Mitsui Norin Co Ltd, Food Res Inst, Lab QC & Food Anal, Shizuoka 4260133, Japan

[2] Natl Inst Genet, Radioisotope Ctr, Shizuoka 4110801, Japan

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2000年 / 46卷 / 01期

关键词：

Bacillaceae; genus Bacillus; grouping; identification; 16S rRNA gene;

D O I：

10.2323/jgam.46.1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5' end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.

引用

页码：1 / 8

页数：8

共 23 条

[1] PHYLOGENETIC HETEROGENEITY OF THE GENUS BACILLUS REVEALED BY COMPARATIVE-ANALYSIS OF SMALL-SUBUNIT-RIBOSOMAL RNA SEQUENCES
ASH, C
FARROW, JAE
WALLBANKS, S
COLLINS, MD
[J]. LETTERS IN APPLIED MICROBIOLOGY, 1991, 13 (04) : 202 - 206
[2] ASH C, 1993, ANTON LEEUW INT J G, V64, P253
[3] FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS
EZAKI, T
HASHIMOTO, Y
YABUUCHI, E
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03): : 224 - 229
[4] Discrimination between Bacillus cereus and Bacillus thuringiensis using specific DNA probes based on variable regions of 16S rRNA
teGiffel, MC
Beumer, RR
Klijn, N
Wagendorp, A
Rombouts, FM
[J]. FEMS MICROBIOLOGY LETTERS, 1997, 146 (01) : 47 - 51
[5] Virgibacillus:: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950).: Emended description of Virgibacillus pantothenticus
Heyndrickx, M
Lebbe, L
Kersters, K
De Vos, P
Forsyth, C
Logan, NA
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 : 99 - 106
[6] IAN H, 1995, FEMS MICROBIOL LETT, V128, P113
[7] Application of the variable region in 16S rDNA to create an index for rapid species identification in the genus Streptomyces
Kataoka, M
Ueda, K
Kudo, T
Seki, T
Yoshida, T
[J]. FEMS MICROBIOLOGY LETTERS, 1997, 151 (02) : 249 - 255
[8] A SIMPLE METHOD FOR ESTIMATING EVOLUTIONARY RATES OF BASE SUBSTITUTIONS THROUGH COMPARATIVE STUDIES OF NUCLEOTIDE-SEQUENCES
KIMURA, M
[J]. JOURNAL OF MOLECULAR EVOLUTION, 1980, 16 (02) : 111 - 120
[9] TAXONOMIC RELATIONSHIP OF BLACK-PIGMENTED BACILLUS-SUBTILIS STRAINS AND A PROPOSAL FOR BACILLUS-ATROPHAEUS SP-NOV
NAKAMURA, LK
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03): : 295 - 300
[10] PHENETIC DIVERSITY OF ALKALIPHILIC BACILLUS STRAINS - PROPOSAL FOR 9 NEW SPECIES
NIELSEN, P
FRITZE, D
PRIEST, FG
[J]. MICROBIOLOGY-UK, 1995, 141 : 1745 - 1761

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