Sequence variability of retroviral particles derived from human melanoma cells - Melanoma-associated retrovirus

被引：12

作者：

Hirschl, Sonja

Schanab, Oliver

Seppele, Heide

Waltenberger, Andrea

Humer, Johannes

Wolff, Klaus

Pehamberger, Hubert

Muster, Thomas

机构：

[1] Univ Vienna, Sch Med, Dept Dermatol, Div Gen Dermatol, Vienna, Austria

[2] Green Hills Biotechnol, Vienna, Austria

来源：

VIRUS RESEARCH | 2007年 / 123卷 / 02期

关键词：

melanoma; HERV; variability;

D O I：

10.1016/j.virusres.2006.08.010

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have shown that melanoma cells produce viral particles that contain sequences which are homologous to human endogenous retroviruses. In this study particles derived from different melanoma cell lines and from melanoma cells of a lymph node metastasis were characterized. We determined the density and the reverse transcriptase (RT) activity of viral particles. Furthermore, we analyzed the sequence variability of multiple clones of each particle preparation. The particles were found to package sequences, which vary for each of the analyzed cell lines. Moreover, even particles derived from the same cell line contain heterologous sequences. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：211 / 215

页数：5

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