Efficient complementation by chimeric Microviridae internal scaffolding proteins is a function of the COOH-terminus of the encoded protein

被引:12
作者
Burch, AD [1 ]
Fane, BA [1 ]
机构
[1] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA
基金
美国国家科学基金会;
关键词
D O I
10.1006/viro.2000.0306
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microviridae morphogenesis is dependent on two scaffolding proteins, an internal and external species. Both structural and genetic analyses suggest that the COOH-terminus of the internal protein is critical for coat protein recognition and specificity. To test this hypothesis, chimeric internal scaffolding genes between Microviridae members phi X174, G4, and alpha 3 were constructed and the proteins expressed in vivo. All of the chimeric proteins were functional in complementation assays. However, the efficient complementation was observed only when the viral coat protein and COOH-terminus of internal scaffolding were of the same origin. Genes with 5' deletions of the phi X174 internal scaffolding gene were also constructed and expressed in vivo. Proteins lacking the first 10 amino acids, which self-associate across the twofold axes of symmetry in the atomic structure, efficiently complement phi X174 am(B) mutants at temperatures above 24 degrees C. These results suggest that internal scaffolding protein self-associations across the twofold axes of symmetry are required only at lower temperatures. (C) 2000 Academic Press.
引用
收藏
页码:286 / 290
页数:5
相关论文
共 16 条
  • [1] BAUDETMILLER M, 1996, J VIROL, V70, P8081
  • [2] INITIATION OF P22-PROCAPSID ASSEMBLY INVIVO
    BAZINET, C
    KING, J
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1988, 202 (01) : 77 - 86
  • [3] Cross-functional analysis of the Microviridae internal scaffolding protein
    Burch, AD
    Ta, J
    Fane, BA
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1999, 286 (01) : 95 - 104
  • [4] Casjens S., 1988, BACTERIOPHAGES, V1, P16
  • [5] The role of scaffolding proteins in the assembly of the small, single-stranded DNA virus φX174
    Dokland, T
    Bernal, RA
    Burch, A
    Pletnev, S
    Fane, BA
    Rossmann, MG
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1999, 288 (04) : 595 - 608
  • [6] Structure of a viral procapsid with molecular scaffolding
    Dokland, T
    McKenna, R
    Ilag, LL
    Bowman, BR
    Incardona, NL
    Fane, BA
    Rossmann, MG
    [J]. NATURE, 1997, 389 (6648) : 308 - 313
  • [7] CHARACTERIZATION OF THE MORPHOGENETIC DEFECTS CONFERRED BY COLD-SENSITIVE PROHEAD ACCESSORY AND SCAFFOLDING PROTEINS OF PHI-X174
    EKECHUKWU, MC
    FANE, BA
    [J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (03) : 829 - 830
  • [8] FANE BA, 1991, GENETICS, V128, P663
  • [9] Hayashi M., 1988, BACTERIOPHAGES, P1
  • [10] GENETIC-CONTROL OF ORGANELLE ASSEMBLY AT THE MOLECULAR-LEVEL .4. SCAFFOLDING PROTEINS AND THE GENETIC-CONTROL OF VIRUS SHELL ASSEMBLY
    KING, J
    GRIFFINSHEA, R
    FULLER, MT
    [J]. QUARTERLY REVIEW OF BIOLOGY, 1980, 55 (04) : 369 - 393