Hypoxia-inducible factor asparaginyl hydroxylase (FIH-1) catalyses hydroxylation at the β-carbon of asparagine-803

被引：178

作者：

McNeill, LA ^{[1
]}

Hewitson, KS ^{[1
]}

Claridge, TD ^{[1
]}

Seibel, JF ^{[1
]}

Horsfall, LE ^{[1
]}

Schofield, CJ ^{[1
]}

机构：

[1] Univ Oxford, Oxford Ctr Mol Sci, Dyson Perrins Lab, Oxford OX1 3QY, England

来源：

BIOCHEMICAL JOURNAL | 2002年 / 367卷 / 03期

关键词：

asparagine hydroxylation; factor inhibiting hypoxia-inducible; factor-1 (FIH-1); hypoxia; oxygenase; oxygen sensing;

D O I：

10.1042/BJ20021162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Asparagine-803 in the C-terminal transactivation domain of human hypoxia-inducible factor (HIF)-1 alpha-subunit is hydroxylated by factor inhibiting HIF-1 (FIH-1) under normoxic conditions causing abrogation of the HIF-1alpha/p300 interaction. NMR and other analyses of a hydroxylated HIF fragment produced in vitro demonstrate that hydroxylation occurs at the beta-carbon of Asn-803 and imply production of the threo-isomer, in contrast with other known aspartic acid/asparagine hydroxylases that produce the erythro-isomer.

引用

页码：571 / 575

页数：5

共 31 条

[1] SPECIFIC CLEAVAGE OF PEPTIDES CONTAINING AN ASPARTIC-ACID (BETA-HYDROXAMIC ACID) RESIDUE [J].

BLODGETT, JK ;

LOUDON, GM ;

COLLINS, KD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (14) :4305-4313

[2] Asymmetric synthesis of orthogonally protected L-threo-β-hydroxyasparagine [J].

Boger, DL ;

Lee, RJ ;

Bounaud, PY ;

Meier, P .

JOURNAL OF ORGANIC CHEMISTRY, 2000, 65 (20) :6770-6772

[3] A conserved family of prolyl-4-hydroxylases that modify HIF [J].

Bruick, RK ;

McKnight, SL .

SCIENCE, 2001, 294 (5545) :1337-1340

[4] Synthesis of 3-hydroxylated analogues of D-aspartic acid beta-hydroxamate [J].

Charvillon, FB ;

Amouroux, R .

SYNTHETIC COMMUNICATIONS, 1997, 27 (03) :395-403

[5] Structural basis for Hif-1α/CBP recognition in the cellular hypoxic response [J].

Dames, SA ;

Martinez-Yamout, M ;

De Guzman, RN ;

Dyson, HJ ;

Wright, PE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5271-5276

[6] C-elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation [J].

Epstein, ACR ;

Gleadle, JM ;

McNeill, LA ;

Hewitson, KS ;

O'Rourke, J ;

Mole, DR ;

Mukherji, M ;

Metzen, E ;

Wilson, MI ;

Dhanda, A ;

Tian, YM ;

Masson, N ;

Hamilton, DL ;

Jaakkola, P ;

Barstead, R ;

Hodgkin, J ;

Maxwell, PH ;

Pugh, CW ;

Schofield, CJ ;

Ratcliffe, PJ .

CELL, 2001, 107 (01) :43-54

[7]

Evans JNS., 1995, BIOMOLECULAR NMR SPE

[8] Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1α [J].

Freedman, SJ ;

Sun, ZYJ ;

Poy, F ;

Kung, AL ;

Livingston, DM ;

Wagner, G ;

Eck, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5367-5372

[9]

GRONKE RS, 1990, J BIOL CHEM, V265, P8558

[10] NOVEL ACCESS TO (3R)-3-HYDROXYL-L-ASPARTIC AND (3S)-3-HYDROXY-L-ASPARTIC ACIDS, (4S)-4-HYDROXY-L-GLUTAMIC ACID, AND RELATED AMINO-ACIDS [J].

HANESSIAN, S ;

VANASSE, B .

CANADIAN JOURNAL OF CHEMISTRY, 1993, 71 (09) :1401-1406

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