Comparison of ELISA and SPR biosensor technology for the detection of paralytic shellfish poisoning toxins

被引：60

作者：

Campbell, Katrina ^{[1
]}

Huet, Anne-Catherine ^{[2
]}

Charlier, Caroline ^{[2
]}

Higgins, Cowan ^{[3
]}

Delahaut, Philippe ^{[2
]}

Elliott, Christopher T. ^{[1
]}

机构：

[1] Queens Univ Belfast, IAFLU, Belfast BT9 5AG, Antrim, North Ireland

[2] Ctr Econ Rurale, B-6900 Marloie, Belgium

[3] Agri Food & Biosci Inst, Belfast BT4 3SD, Antrim, North Ireland

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 32期

关键词：

Enzyme labeled immunosorbent assay (ELISA); Biosensor; Surface plasmon resonance (SPR); Paralytic shellfish poisoning (PSP) toxin; Polyclonal antibody; SURFACE-PLASMON RESONANCE; LINKED-IMMUNOSORBENT-ASSAY; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUOROMETRIC MICROPLATE ASSAY; FLIGHT MASS-SPECTROMETRY; PRECHROMATOGRAPHIC OXIDATION; QUANTITATIVE-DETERMINATION; FLUORESCENCE DETECTION; ENZYME-IMMUNOASSAY; MOUSE BIOASSAY;

D O I：

10.1016/j.jchromb.2009.10.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme labeled immunosorbent assay (ELISA) and surface plasmon resonance (SPR) biosensor assay for the detection of paralytic shellfish poisoning (PSP) toxins were developed and a comparative evaluation was performed. A polyclonal antibody (BC67) used in both assay formats was raised to saxitoxin-jeffamine-BSA in New Zealand white rabbits. Each assay format was designed as an inhibition assay. Shellfish samples (n = 54) were evaluated by each method using two simple rapid extraction procedures and compared to the AOAC high performance liquid chromatography (HPLC) and the mouse bioassay (MBA). The results of each assay format were comparable with the HPLC and MBA methods and demonstrate that an antibody with high sensitivity and broad specificity to PSP toxins can be applied to different immunological techniques. The method of choice will depend on the end-users needs. The reduced manual labor and simplicity of operation of the SPR biosensor compared to ELISA, ease of sample extraction and superior real time semi-quantitative analysis are key features that could make this technology applicable in a high-throughput monitoring unit. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：4079 / 4089

页数：11

共 51 条

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