Sphingosylphosphorylcholine induces endothelial cell migration and morphogenesis

被引:66
作者
Boguslawski, G
Lyons, D
Harvey, KA
Kovala, AT
English, D [1 ]
机构
[1] Clarian Hlth Partners Inc, Methodist Res Inst, Expt Cell Res Program, Indianapolis, IN 46202 USA
[2] Indiana Univ, Sch Med, Dept Physiol & Biophys, Indianapolis, IN 46202 USA
关键词
endothelial cells; sphingosylphosphorylcholine; sphingosine; 1-phosphate; angiogenesis; chemotaxis; Edg1 receptor RNA; Matrigel assay; capillaries;
D O I
10.1006/bbrc.2000.2822
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sphingosylphosphorylcholine (SPC) is one of the biologically active phospholipids that may act as extracellular messengers. Particularly important is the role of these lipids in the angiogenic response, a complex process involving endothelial cell migration, proliferation, and morphologic differentiation. Here we demonstrate that SPC and its hydrolytic product, sphingosine, induce chemotactic migration of human and bovine endothelial cells. The response is approximately equal to that elicited by vascular endothelial cell growth factor. The effect of SPC and sphingosine was associated with a rapid down-regulation of Edg1, a sphingosine 1-phosphate (SPP)-specific receptor involved in endothelial cell chemotaxis. Both SPC and sphingosine induced differentiation of endothelial cells into capillary-like structures in vitro. Thus, SPC and sphingosine join SPP among the biologically active lipids with angiogenic potential. Since neuronal abnormalities accompany pathological accumulation of SPC in brain tissue, it is possible that SPC is a modulator of angiogenesis in neural tissue upon its release from brain cells following trauma or neoplastic growth. (C) 2000 Academic Press.
引用
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页码:603 / 609
页数:7
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