Secretion of the Caulobacter crescentus S-layer protein:: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins
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作者:
Bingle, WH
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Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, CanadaUniv British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
Bingle, WH
[1
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Nomellini, JF
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Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, CanadaUniv British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
Nomellini, JF
[1
]
Smit, J
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Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, CanadaUniv British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
Smit, J
[1
]
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[1] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies showed that 336 or 242 C-terminal residues of RsaA mediated secretion of >50 mg of a cellulase passenger protein per liter to the culture fluids.