Secretion of the Caulobacter crescentus S-layer protein:: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins

被引:41
作者
Bingle, WH [1 ]
Nomellini, JF [1 ]
Smit, J [1 ]
机构
[1] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada
关键词
D O I
10.1128/JB.182.11.3298-3301.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies showed that 336 or 242 C-terminal residues of RsaA mediated secretion of >50 mg of a cellulase passenger protein per liter to the culture fluids.
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页码:3298 / 3301
页数:4
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