Cre-loxP-mediated bax gene activation reduces growth rate and increases sensitivity to chemotherapeutic agents in human gastric cancer cells

被引：12

作者：

Komatsu, K

Suzuki, S

Shimosegawa, T

Miyazaki, J

Toyota, T

机构：

[1] Tohoku Univ, Sch Med, Dept Internal Med 3, Sendai, Miyagi 980, Japan

[2] Osaka Univ, Sch Med, Dept Physiol Chem & Nutr, Osaka, Japan

来源：

CANCER GENE THERAPY | 2000年 / 7卷 / 06期

关键词：

bax; Cre-loxP system; chemotherapy; Bcl-2; apoptosis;

D O I：

10.1038/sj.cgt.7700181

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dysregulation of apoptosis may be closely related to the development of cancer and its chemoresistance. Overexpression of Bax, an inducer of apoptosis, has led to increased cell death in a variety of cancer cell lines. In this study, we investigated the effect of Bax overexpression in two gastric cancer cell lines, MKN-28 and MKN-45, using a Cre-loxP-mediated inducible expression system. After induction of bax, both cell lines showed decreased proliferation, partially due to increased cell death. Furthermore, Bax-expressing MKN-28 cells were more sensitive to cisplatin. These results indicate that up-regulation of the bax gene may provide a novel strategy for the treatment of gastric cancer.

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页码：885 / 892

页数：8

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