The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

被引：50

作者：

Zhang, XX ^{[1
]}

Turner, SL

Guo, XW

Yang, HJ

Debelló, F

Yang, GP

Dénarié, J

Young, JPW

Li, FD

机构：

[1] Huazhong Agr Univ, Dept Microbiol, Wuhan 430070, Peoples R China

[2] INRA, CNRS, Lab Biol Mol Relat Plantes Microorganismes, F-31326 Castanet Tolosan, France

[3] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 07期

关键词：

D O I：

10.1128/AEM.66.7.2988-2995.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nodulation genes of Mesorhizobium sp, (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nonDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria.

引用

页码：2988 / 2995

页数：8

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