Enhancer trap expression patterns provide a novel teaching resource

被引:16
作者
Geisler, M [1 ]
Jablonska, B [1 ]
Springer, PS [1 ]
机构
[1] Univ Calif Riverside, Ctr Plant Cell Biol, Dept Bot & Plant Sci, Riverside, CA 92521 USA
关键词
D O I
10.1104/pp.011197
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A collection of Arabidopsis enhancer trap transposants has been identified for use as a teaching tool. This collection serves to assist students in understanding the patterning and organization of plant tissues and cells, and will be useful in plant anatomy, morphology, and developmental biology courses. Each transposant exhibits reporter gene expression in a specific tissue, cell type, or domain, and these lines collectively offer a glimpse of compartments of gene expression. Some compartments correspond to classical definitions of botanical anatomy and can assist in anatomical identification. Other patterns of reporter gene expression are more complex and do not necessarily correspond to known anatomical features. The sensitivity of the beta-glucuronidase histochemical stain provides the student with a colorful and direct way to visualize difficult aspects of plant development and anatomy, and provides the teacher with an invaluable tool for a practical laboratory session.
引用
收藏
页码:1747 / 1753
页数:7
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