Chimeric swine vesicular disease viruses produced by fusion PCR: a new method for epitope mapping

被引：11

作者：

Dekker, A ^{[1
]}

Leendertse, CH ^{[1
]}

van Poelwijk, F ^{[1
]}

Rebel, JMJ ^{[1
]}

Moormann, RJM ^{[1
]}

机构：

[1] Inst Anim Sci & Hlth, DLO, ID, Dept Mammalian Virol, NL-8200 AB Lelystad, Netherlands

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 86卷 / 02期

关键词：

epitope mapping; monoclonal antibodies; chimeric virus; picornavirus; swine vesicular disease;

D O I：

10.1016/S0166-0934(00)00142-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method of epitope mapping based on chimeric swine vesicular disease (SVD) viruses produced by fusion PCR (polymerase chain reaction). Seven out of 16 neutralising and non-neutralising newly produced monoclonal antibodies (MAbs) discriminated between SVD isolate ITL/1/66 and NET/1/92. Using fusion PCR eight chimeric viruses were produced containing different supplementary pieces of the P1 region of both parent strains. Using these chimeric viruses we were able to map the the epitope regions recognised by these seven neutralising and non-neutralising Mabs. This new method, using chimeric viruses produced by fusion PCR, is particularly valuable for the epitope mapping of non-neutralising MAbs. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：131 / 141

页数：11

共 22 条

[1] DEVELOPMENT OF 2 NOVEL MONOCLONAL ANTIBODY-BASED ELISAS FOR THE DETECTION OF ANTIBODIES AND THE IDENTIFICATION OF SWINE ISOTYPES AGAINST SWINE VESICULAR DISEASE VIRUS [J].

BROCCHI, E ;

BERLINZANI, A ;

GAMBA, D ;

DESIMONE, F .

JOURNAL OF VIROLOGICAL METHODS, 1995, 52 (1-2) :155-167

[2]

BROECHI E, 1997, EPIDEMIOL INFECT, V118, P51

[3] ANTIGENIC DIFFERENCES BETWEEN ISOLATES OF SWINE VESICULAR DISEASE VIRUS AND THEIR RELATIONSHIP TO COXSACKIE B5 VIRUS [J].

BROWN, F ;

TALBOT, P ;

BURROWS, R .

NATURE, 1973, 245 (5424) :315-316

[4] Validation of a monoclonal antibody-based ELISA to detect antibodies directed against swine vesicular disease virus [J].

Chénard, G ;

Bloemraad, M ;

Kramps, JA ;

Terpstra, C ;

Dekker, A .

JOURNAL OF VIROLOGICAL METHODS, 1998, 75 (01) :105-112

[5] IDENTIFICATION OF A 5TH NEUTRALIZABLE SITE ON TYPE-O FOOT-AND-MOUTH-DISEASE VIRUS FOLLOWING CHARACTERIZATION OF SINGLE AND QUINTUPLE MONOCLONAL-ANTIBODY ESCAPE MUTANTS [J].

CROWTHER, JR ;

FARIAS, S ;

CARPENTER, WC ;

SAMUEL, AR .

JOURNAL OF GENERAL VIROLOGY, 1993, 74 :1547-1553

[6] VALIDATION OF A SCREENING LIQUID-PHASE BLOCKING ELISA FOR SWINE VESICULAR DISEASE [J].

DEKKER, A ;

MOONEN, PLJM ;

TERPSTRA, C .

JOURNAL OF VIROLOGICAL METHODS, 1995, 51 (2-3) :343-348

[7]

FAZEKAS S, 1980, J IMMUNOL METHODS, V35, P1

[8]

Franssen P. G. J., 1975, Tijdschrift voor Diergeneeskunde, V100, P1325

[9]

GENNIP HGP, 1999, J VIROL METHODS, V78, P117

[10] SEROLOGICAL RELATIONSHIP OF SWINE VESICULAR DISEASE VIRUS AND COXSACKIE B5 VIRUS [J].

GRAVES, JH .

NATURE, 1973, 245 (5424) :314-315

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