Quorum-sensing-directed protein expression in Serratia proteamaculans B5a

被引:105
作者
Christensen, AB
Riedel, K
Eberl, L
Flodgaard, LR
Molin, S
Gram, L
Givskov, M
机构
[1] Tech Univ Denmark, BioCentrum DTU, Sect Mol Microbiol, DK-2800 Lyngby, Denmark
[2] Tech Univ Munich, Lehrstuhl Mikrobiol, D-85350 Freising Weihenstephan, Germany
[3] Tech Univ Denmark, Dept Seafood Res, Danish Inst Fisheries Res, DK-2800 Lyngby, Denmark
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.25575-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
N-Acyl-L-homoserine-lactone-producing Serratia species are frequently encountered in spoiling foods of vegetable and protein origin. The role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. A set of luxR luxI homologous genes encoding a putative quorum sensor was identified in the N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL)-producing Serratia proteamaculans strain B5a. The 3-oxo-C6-HSL synthase SprI showed 79% similarity with Esal from Pantoea stewartii and the putative regulatory protein SprR was 86% similar to the SpnR of Serratia marcescens. Proteome analysis suggested that the presence of at least 39 intracellular proteins was affected by the 3-oxo-C6-HSL-based quorum sensing system. The lipB-encoded secretion system was identified as one target gene of the quorum sensing system. LipB was required for the production of extracellular lipolytic and proteolytic activities, thus rendering the production of food-deterioration-relevant exoenzymes indirectly under the control of quorum sensing. Strain B5a caused quorum-sensing-controlled spoilage of milk. Furthermore, chitinolytic activity was controlled by quorum sensing. This control appeared to be direct and not mediated via LipB. The data presented here demonstrate that quorum-sensing-controlled exoenzymic activities affect food quality.
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页码:471 / 483
页数:13
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