Keratinolytic proteases of Bacillus species isolated from the Amazon basin showing remarkable de-hairing activity

被引：56

作者：

Giongo, Janice Luhering

Lucas, Francoise S.

Casarin, Franciani

Heeb, Philipp

Brandelli, Adriano

机构：

[1] Univ Fed Rio Grande do Sul, Lab Bioquim & Microbiol Aplicada, ICTA, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland

[3] UPVM, Fac Sci & Technol, F-94010 Creteil, France

[4] Univ Toulouse 3, Lab Evolut & Divers Biol, F-31062 Toulouse 9, France

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 23卷 / 03期

关键词：

Bacillus; protease; keratinase; feather; leather; depilatory activity;

D O I：

10.1007/s11274-006-9234-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three keratinolytic Bacillus spp. isolated from the Brazilian Amazon basin were characterized. The strains P6, P7 and P11 were identified based on morphological and biochemical characteristics and 16S rDNA sequences. P6, P7 and P11 sequences shared more than 99% similarity with B. subtilis, B. amyloliquefaciens and B. velesensis. The keratinases produced by these bacteria were active on azokeratin and degradation of feather barbules was observed. The enzymes were inhibited by the serine protease inhibitor PMSF, and showed maximum activity at pH 9.0. Proteins like albumin, casein and gelatin were hydrolysed by these keratinases. Depilatory studies on bovine pelts revealed that all three strains were efficient in promoting de-hairing. Microscopic analysis showed that the epidermis was completely removed and the absence of hair in follicles was observed.

引用

页码：375 / 382

页数：8

共 42 条

[1]

[Anonymous], 1989, Cladistics, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X

[2] Alkaline proteases: A review [J].

Anwar, A ;

Saleemuddin, M .

BIORESOURCE TECHNOLOGY, 1998, 64 (03) :175-183

[3] Salt-tolerant phenol-degrading microorganisms isolated from Amazonian soil samples [J].

Ribeiro Bastos A.E. ;

Moon D.H. ;

Rossi A. ;

Trevors J.T. ;

Tsai S.M. .

Archives of Microbiology, 2000, 174 (5) :346-352

[4] Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojavensis [J].

Beg, QK ;

Gupta, R .

ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (02) :294-304

[5] Bacterial carbon metabolism in the Amazon River system [J].

Benner, R ;

Opsahl, S ;

ChinLeo, G ;

Richey, JE ;

Forsberg, BR .

LIMNOLOGY AND OCEANOGRAPHY, 1995, 40 (07) :1262-1270

[6]

BOCKLE B, 1995, APPL ENVIRON MICROB, V61, P3705

[7] Molecular microbial diversity in soils from eastern Amazonia: Evidence for unusual microorganisms and microbial population shifts associated with deforestation [J].

Borneman, J ;

Triplett, EW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (07) :2647-2653

[8]

Bressollier P, 1999, APPL ENVIRON MICROB, V65, P2570

[9] Feather meals and meat and bone meals from different origins as protein sources in rainbow trout (Oncorhynchus mykiss) diets [J].

Bureau, DP ;

Harris, AM ;

Bevan, DJ ;

Simmons, LA ;

Azevedo, PA ;

Cho, CY .

AQUACULTURE, 2000, 181 (3-4) :281-291

[10] BanAI a new isoschizomer of the type II restriction endonuclease HaeIII discovered in a Bacillus anthracis isolate from Amazon Basin [J].

Chies, JM ;

Dias, ACD ;

Maia, HMM ;

Astolfi, S .

FEMS MICROBIOLOGY LETTERS, 2002, 215 (01) :97-101

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