Cloning and characterization of the Neisseria meningitidis rfaC gene encoding alpha-1,5 heptosyltransferase I

被引：20

作者：

Stojiljkovic, I

Hwa, V

Larson, J

Lin, L

So, M

Nassif, X

机构：

[1] UNIV PARIS 05,FAC MED NECKER ENFANTS MALAD,INSERM,U411,LAB MICROBIOL,PARIS,FRANCE

[2] EMORY UNIV,DEPT IMMUNOL & MICROBIOL,ATLANTA,GA 30322

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 151卷 / 01期

关键词：

Neisseria meningitidis; lipooligosaccharide; rfaC;

D O I：

10.1016/S0378-1097(97)00135-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We cloned and characterized the Neisseria meningitidis rfaC gene which encodes an enzyme, alpha-1,5 heptosyltransferase I, involved in the synthesis of the deep-core of the lipooligosaccharide, The N. meningitidis rfaC mutant, obtained by an allelic exchange, produced lipooligosaccharide which migrated faster in sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the lipooligosaccharide isolated from the wird-type N. meningitidis. The N. meningitidis rfaC mutant was not affected by growth in a rich microbiological medium and did not show any defect in adhesion to epithelial cell lines. Conversely, the rfaC mutant was attenuated in the infant rat model of meningococcemia, thus confirming the importance of intact lipooligosaccharide in the Virulence of N. meningitidis.

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页码：41 / 49

页数：9

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