Towards obtaining ES cells in the marine fish species Sparus aurata;: multipassage maintenance, characterization and transfection

被引：17

作者：

Béjar, J

Hong, Y

Alvarez, MC ^{[1
]}

机构：

[1] Univ Malaga, Fac Ciencias, Dept Genet, E-29071 Malaga, Spain

[2] Univ Wurzburg, Bioctr, D-97074 Wurzburg, Germany

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 15卷 / 3-5期

关键词：

Sparus aurata; seabream; embryonic stem cells; transgenic fish;

D O I：

10.1016/S1050-3862(99)00015-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Animal Embryonic-Stem (ES) cells represents a unique tool in animal genetic manipulation. Though putative ES cells from several species have been reported, only those from mice proved successful. In this work, a long-term embryonic cell culture, derived from the commercial fish (Sparus aurata), is reported. These cells have been in vitro characterized for totipotency and transfected with a GFP plasmid, (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：125 / 129

页数：5

共 23 条

[1] SHORT-TERM CELL-CULTURE TECHNIQUE FOR OBTAINING CHROMOSOMES IN MARINE AND FRESH-WATER FISH [J].