Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy

被引:80
作者
Böttcher, C
Ludwig, K
Herrmann, A
van Heel, M
Stark, H
机构
[1] Max Planck Gesell, Fritz Haber Inst, D-14195 Berlin, Germany
[2] Free Univ Berlin, Inst Chem, Forschungszentrum Elektronenmikroskopie, D-14195 Berlin, Germany
[3] Humboldt Univ, Inst Biol Biophys, Math Nat Fak 1, D-10115 Berlin, Germany
[4] Univ London Imperial Coll Sci Technol & Med, Wolfson Labs, Dept Biochem, London SW7 2AY, England
[5] Inst Mol Biol & Tumorforsch, D-35037 Marburg, Germany
来源
FEBS LETTERS | 1999年 / 463卷 / 03期
关键词
electron microscopy; fusion; influenza; hemagglutinin; structure;
D O I
10.1016/S0014-5793(99)01475-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The three-dimensional structures of the complete haemagglutinin (MA) of influenza virus A/Japan/305/57 (H2N2) in its native (neutral pH) and membrane fusion-competent (low pH) form by electron cryo-microscopy at resolution of 10 Angstrom and 14 Angstrom, respectively, have been determined, In the fusion-competent form the subunits remain closely. associated preserving typical overall features of the trimeric ectodomain at neutral pH. Rearrangements of the tertiary structure in the distal and the stent parts are associated with the formation of a central cavity through the entire ectodomain, We suggest that the cavity is essential for relocation of the so-called fusion sequence of HA towards the target membrane. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:255 / 259
页数:5
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