Detection of bacteria by surface-enhanced Raman spectroscopy

被引：160

作者：

Sengupta, Atanu

Mujacic, Mirna

Davis, E. James

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 386卷 / 05期

基金：

美国国家科学基金会;

关键词：

bacteria detection; bacterial pathogens; Raman spectroscopy;

D O I：

10.1007/s00216-006-0711-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection and identification of dilute bacterial samples by surface-enhanced Raman spectroscopy has been explored by mixing aqueous suspensions of bacteria with a suspension of nanocolloidal silver particles. An estimate of the detection limit of E. coli was obtained by varying the concentration of bacteria. By correcting the Raman spectra for the broad librational OH band of water, reproducible spectra were obtained for E. coli concentrations as low as approximately 10(3) cfu/mL. To aid in the assignment of Raman bands, spectra for E. coli in D2O are also reported.

引用

页码：1379 / 1386

页数：8

共 29 条

[1] The effect of a sharp pycnocline on plankton dynamics in a freshwater influenced Norwegian fjord [J].