Expression of drug-metabolizing enzymes

被引：31

作者：

Guengerich, FP ^{[1
]}

Parikh, A ^{[1
]}

机构：

[1] VANDERBILT UNIV, MED CTR, SCH MED, CTR MOL TOXICOL, NASHVILLE, TN 37232 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0958-1669(97)80039-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A principal advance in the production of drug-metabolizing enzymes has been the development of catalytically self-sufficient cytochrome P450 systems, including additional P450-reductase fusion proteins and Escherichia coli and baculovirus coexpression constructs. Continuing work with glutathione transferases has resulted in the identification of important residues by random mutagenesis screening techniques, as well as in the engineering of model Salmonella typhimurium strains for genotoxicity analysis.

引用

页码：623 / 628

页数：6

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