A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control

被引:88
作者
Pridgeon, Julia W. [1 ]
Becnel, James J. [1 ]
Clark, Gary C. [1 ]
Linthicum, Kenneth J. [1 ]
机构
[1] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA
关键词
high-throughput screen; pesticides; mosquito control; AEDES-AEGYPTI DIPTERA; YELLOW-FEVER VIRUS; CULEX-QUINQUEFASCIATUS; TRANSMISSION; CULICIDAE; DENGUE; RESISTANCE; FILARIASIS;
D O I
10.1603/033.046.0219
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC(50) values of a representative group of compounds were determined using this high-throughput screening method and compared with LD(50) values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.
引用
收藏
页码:335 / 341
页数:7
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