SNPs on chips: The hidden genetic code in expression arrays

被引：17

作者：

Sliwerska, Elzbieta

Meng, Fan

Speed, Terence P.

Jones, Edward G.

Bunney, William E.

Akil, Huda

Watson, Stanley J.

Burmeister, Margit

机构：

[1] Univ Michigan, Mol & Behav Neurosci Inst, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Psychiat, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA

[4] Univ Calif Berkeley, Dept Stat, Berkeley, CA 94720 USA

[5] Univ Calif Davis, Dept Psychiat, Davis, CA 95616 USA

来源：

BIOLOGICAL PSYCHIATRY | 2007年 / 61卷 / 01期

关键词：

Affymetrix; Catechol-O-Methyl-Transferase; gene expression; genetic association; microarray; postmortem brain;

D O I：

10.1016/j.biopsych.2006.01.023

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Gene expression microarray analysis in postmortem brains is one of the fastest growing fields of psychiatric research. Here we show that common polymorphisms (SNTs) present on probe sets can masquerade as significant "gene expression" differences. After first observing this artifact in the Catechol-O-methyl transterase (COMT) gene, we replicate the finding in two additional genes predicted to show this artifact. Many Affymetrix chips contain thousands of SNPs that are both common and in the central probe region affecting hybridization, and thus have the potential to confound expression analysis.

引用

页码：13 / 16

页数：4

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