Oleosin gene expression in olive

被引：21

作者：

Giannoulia, Katerina ^{[1
]}

Banilas, Georgios ^{[1
]}

Hatzopoulos, Polydefkis ^{[1
]}

机构：

[1] Agr Univ Athens, Dept Agr Biotechnol, Mol Biol Lab, Athens 11855, Greece

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2007年 / 164卷 / 01期

关键词：

developmental regulation; embryo; endosperm; oleosin; olive;

D O I：

10.1016/j.jplph.2006.03.016

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

To study the spatial and temporal regulation of oleosin gene expression during olive drupe development, a cDNA (OeOLE) was isolated from embryos. The deduced amino acid sequence of 165 amino acid residues exhibits a tong central hydrophobic stretch, including the conserved "proline knot" motif. Phylogenetic analysis grouped OeOLE into the class of high (H) molecular weight oleosins. Southern blotting indicated that the gene is represented by 1-2 copies in the olive genome. Transcript analysis revealed that OeOLE is expressed solely in seeds. A similar bell-shaped pattern of expression was observed in both embryos and endosperms. Transcript accumulation starts at late heart embryo stage, reaches maximum levels at mid-torpedo stage and thereafter declines, coinciding the stages of most oil accumulation in those tissues. (c) 2006 Elsevier GmbH. All rights reserved.

引用

页码：104 / 107

页数：4

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