Enhanced microfiltration of γ-globulin solution upon treatment of NaCl addition and/or DNase digestion

被引：10

作者：

Higuchi, A

Nemoto, M

Koyama, H

Hirano, K

Yoon, BO

Hara, M

Yokogi, M

Manabe, SI

机构：

[1] Seikei Univ, Dept Appl Chem, Tokyo 1808633, Japan

[2] Asahi Med Co Ltd, Strateg Technol & Dev, Chiyoda Ku, Tokyo 1018481, Japan

[3] Fukuoka Womens Univ, Fac Human Environm Sci, Higashi Ku, Fukuoka 8138529, Japan

来源：

JOURNAL OF MEMBRANE SCIENCE | 2002年 / 210卷 / 02期

关键词：

microfiltration; fouling; globulin; DNase; DNA;

D O I：

10.1016/S0376-7388(02)00417-9

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Microfiltration of a gamma-globulin solution has been investigated through the virus removal membranes having different pore sizes (i.e. r = 15, 35 and 75 nm) and a dialysis membrane (r = 3.4 mm), which were all made of the same regenerated cellulose material. The addition of NaCl in the gamma-globulin feed solution was effective to enhance the flux and transmission through the membranes having a pore size ranging from 15 to 75 nm. DNase treatment of a gamma-globulin solution with Micrococcal nuclease enhanced the flux and transmission of gamma-globulin through the membranes either with or without NaCl. The membranes having a pore size of 35 nm showed dramatically enhanced flux in the microfiltration of a gamma-globulin solution containing NaCl and/or being treated with Micrococcal nuclease. This can be explained as a DNase treatment and NaCl addition in the protein solution dissociate protein aggregates of DNA-gamma-globulin complex, which plugs the pores in the microfiltration membranes. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：369 / 378

页数：10

共 31 条

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