Mouse type-2 retinaldehyde dehydrogenase (RALDH2): genomic organization, tissue-dependent expression, chromosome assignment and comparison to other types

被引:23
作者
Hsu, LC [1 ]
Chang, WC [1 ]
Yoshida, A [1 ]
机构
[1] City Hope Natl Med Ctr, Beckman Res Inst, Div Mol Med, Duarte, CA 91010 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1492卷 / 01期
关键词
gene structure; Raldh2; chromosome assignment; tissue distribution;
D O I
10.1016/S0167-4781(00)00108-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Retinaldehyde dehydrogenase (RALDH) isozymes catalyze the formation of an essential developmental modulator, retinoic acid. We determined the structural organization of mouse type-2 Raldh2 by isolation of overlapping genomic DNA clones from a phage library. The gene consists of 14 exons spanning more than 70 kb of genomic DNA. It was localized to mouse chromosome 6. Northern blot analysis revealed testis-specific expression. The RALDH genes belong to the aldehyde dehydrogenase (ALDH) multi-gene family. Three types of RALDH genes (e.g. human ALDH1/mouse Ahd2/rat RalDH(I), human ALDH11/mouse Raldh2/rat RalDH(II) and human ALDH6) are highly conserved during evolution, sharing about 70% identity at the amino acid level between any two gene types and 90% identity between any two mammalian genes of the same type. Different RALDH types show specific tissue and developmental expression patterns, suggesting (i) a regulatory mechanism of retinoic acid synthesis via different promoters of RALDH genes, and (ii) distinctive biological roles of different isozymes in embryogenesis and organogenesis. (C) 2000 Published by Elsevier Science B.V.
引用
收藏
页码:289 / 293
页数:5
相关论文
共 22 条
[1]   Cloning of a cDNA encoding rat aldehyde dehydrogenase with high activity for retinal oxidation [J].
Bhat, PV ;
Labrecque, J ;
Boutin, JM ;
Lacroix, A ;
Yoshida, A .
GENE, 1995, 166 (02) :303-306
[2]   Changing patterns of renal retinal dehydrogenase expression parallel nephron development in the rat [J].
Bhat, PV ;
Marcinkiewicz, M ;
Li, Y ;
Mader, S .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1998, 46 (09) :1025-1032
[3]  
Haselbeck RJ, 1999, DEV GENET, V25, P353, DOI 10.1002/(SICI)1520-6408(1999)25:4<353::AID-DVG9>3.0.CO
[4]  
2-G
[5]   Molecular analysis of two closely related mouse aldehyde dehydrogenase genes:: identification of a role for Aldh1, but not Aldh-pb, in the biosynthesis of retinoic acid [J].
Hsu, LC ;
Chang, WC ;
Hoffmann, I ;
Duester, G .
BIOCHEMICAL JOURNAL, 1999, 339 :387-395
[6]   MOLECULAR-CLONING, GENOMIC ORGANIZATION, AND CHROMOSOMAL LOCALIZATION OF AN ADDITIONAL HUMAN ALDEHYDE DEHYDROGENASE GENE, ALDH6 [J].
HSU, LC ;
CHANG, WC ;
HIRAOKA, L ;
HSIEH, CL .
GENOMICS, 1994, 24 (02) :333-341
[7]   GENOMIC STRUCTURE OF THE HUMAN CYTOSOLIC ALDEHYDE DEHYDROGENASE GENE [J].
HSU, LC ;
CHANG, WC ;
YOSHIDA, A .
GENOMICS, 1989, 5 (04) :857-865
[8]   CLONING OF CDNAS FOR HUMAN ALDEHYDE DEHYDROGENASE-1 AND DEHYDROGENASE-2 [J].
HSU, LC ;
TANI, K ;
FUJIYOSHI, T ;
KURACHI, K ;
YOSHIDA, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (11) :3771-3775
[9]  
King G, 1997, ADV EXP MED BIOL, V414, P19
[10]   The cytosolic aldehyde dehydrogenase gene (Aldh1) is developmentally expressed in Leydig cells [J].
LopezFernandez, LA ;
delMazo, J .
FEBS LETTERS, 1997, 407 (02) :225-229