Modulation of Lon protease activity and aconitase turnover during aging and oxidative stress

被引:194
作者
Bota, DA
Van Remmen, H
Davies, KJA
机构
[1] Univ So Calif, Ethel Percy Andrus Gerontol Ctr, Los Angeles, CA 90089 USA
[2] Univ So Calif, Div Mol & Computat Biol, Los Angeles, CA 90089 USA
[3] Univ Texas, Hlth Sci Ctr, Dept Physiol, San Antonio, TX 78229 USA
关键词
Lon protease; aconitase; protein oxidation; mitochondria; aging; Sod2;
D O I
10.1016/S0014-5793(02)03638-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We compared Lon protease expression in murine skeletal muscle of young and old, wild-type and Sod2(-/+) heterozygous mice, and studied Lon involvement in the accumulation of damaged (oxidized) proteins. Lon protease protein levels were lower in old and oxidatively challenged animals, and this Lon deficiency was associated with increased levels of carbonylated proteins. We identified one of these proteins as aconitase, and another as an aconitase fragmentation product, which we can also generate in vitro by treating purified aconitase with H2O2. These results imply that aging and oxidative stress down-regulate Lon protease expression which, in turn, may be responsible for the accumulation of damaged proteins, such as aconitase, within mitochondria. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:103 / 106
页数:4
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