High occurrence of esp among ampicillin-resistant and vancomycin-susceptible Enterococcus faecium clones from hospitalized patients

被引:52
作者
Coque, TM
Willems, R
Cantón, R
Del Campo, R
Baquero, F
机构
[1] Hosp Ramon y Cajal, Microbiol Serv, E-28034 Madrid, Spain
[2] Natl Inst Publ Hlth & Environm RIVM, Res Lab Infect Dis LIO, Bilthoven, Netherlands
关键词
esp; Enterococcus faecium; ampicillin;
D O I
10.1093/jac/dkf229
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The ability to colonize patients successfully may be essential for the emergence and spread of resistant nosocomial strains. We determined the presence of Esp, a surface protein involved in colonization ability in Enterococcus faecalis, in 96 Enterococcus faecium isolates from hospitalized patients (77 PFGE clones), 33 faecal isolates from healthy volunteers (32 clones) and 20 environmental isolates (20 clones). Esp was found significantly more often in E. faecium isolated from hospitalized patients than in isolates from the community setting (26% versus 6%, P < 0.01) and was significantly more common among ampicillin-resistant than among ampicillin-susceptible strains (37% versus 4%, P < 0.001), regardless of the isolation site. The frequency of the esp gene in the hospital clearly correlates with antibiotic-resistant E. faecium clones. This observation indicates that antibiotic-resistant variants may frequently arise under antibiotic selective pressure among esp-positive clones reaching ecological abundance in the nosocomial habitat.
引用
收藏
页码:1035 / 1038
页数:4
相关论文
共 10 条
[1]   Variant esp gene in vancomycin-sensitive Enterococcus faecium [J].
Baldassarri, L ;
Bertuccini, L ;
Ammendolia, MG ;
Gherardi, G ;
Creti, R .
LANCET, 2001, 357 (9270) :1802-1802
[2]  
Coque TM, 1998, METH MOLEC MED, V15, P469, DOI 10.1385/0-89603-498-4:469
[3]   RETRACTED: A link between virulence and ecological abundance in natural populations of Staphylococcus aureus (Retracted article. See vol 295, pg 971, 2002) [J].
Day, NPJ ;
Moore, CE ;
Enright, MC ;
Berendt, AR ;
Smith, JM ;
Murphy, MF ;
Peacock, SJ ;
Spratt, BG ;
Feil, EJ .
SCIENCE, 2001, 292 (5514) :114-116
[4]   Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection [J].
Shankar, N ;
Lockatell, CV ;
Baghdayan, AS ;
Drachenberg, C ;
Gilmore, MS ;
Johnson, DE .
INFECTION AND IMMUNITY, 2001, 69 (07) :4366-4372
[5]   vanA and vanB incorporate into an endemic ampicillin-resistant vancomycin-sensitive Enterococcus faecium strain:: Effect on interpretation of clonality [J].
Suppola, JP ;
Kolho, E ;
Salmenlinna, S ;
Tarkka, E ;
Vuopio-Varkila, J ;
Vaara, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :3934-3939
[6]   INTERPRETING CHROMOSOMAL DNA RESTRICTION PATTERNS PRODUCED BY PULSED-FIELD GEL-ELECTROPHORESIS - CRITERIA FOR BACTERIAL STRAIN TYPING [J].
TENOVER, FC ;
ARBEIT, RD ;
GOERING, RV ;
MICKELSEN, PA ;
MURRAY, BE ;
PERSING, DH ;
SWAMINATHAN, B .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (09) :2233-2239
[7]   The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation [J].
Toledo-Arana, A ;
Valle, J ;
Solano, C ;
Arrizubieta, MJ ;
Cucarella, C ;
Lamata, M ;
Amorena, B ;
Leiva, J ;
Penadés, JR ;
Lasa, I .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (10) :4538-4545
[8]   Genogrouping and incidence of virulence factors of Enterococcus faecalis in liver transplant patients differ from blood culture and fecal isolates [J].
Waar, K ;
Muscholl-Silberhorn, AB ;
Willems, RJL ;
Slooff, MJH ;
Harmsen, HJM ;
Degener, JE .
JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (08) :1121-1127
[9]   Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals [J].
Willems, RJL ;
Homan, W ;
Top, J ;
van Santen-Verheuvel, M ;
Tribe, D ;
Manzioros, X ;
Gaillard, C ;
Vandenbroucke-Grauls, CMJE ;
Mascini, EM ;
van Kregten, E ;
van Embden, JDA ;
Bonten, MJM .
LANCET, 2001, 357 (9259) :853-855
[10]   Frequency of esp in Enterococcus faeclum isolates [J].
Woodford, N ;
Soltani, M ;
Hardy, KJ .
LANCET, 2001, 358 (9281) :584-584