Normalization of boutique two-color microarrays with a high proportion of differentially expressed probes

被引：52

作者：

Oshlack, Alicia ^{[1
]}

Emslie, Dianne ^{[1
]}

Corcoran, Lynn M. ^{[1
]}

Smyth, Gordon K. ^{[1
]}

机构：

[1] Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Parkville, Vic 3050, Australia

来源：

GENOME BIOLOGY | 2007年 / 8卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1186/gb-2007-8-1-r2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Normalization is critical for removing systematic variation from microarray data. For two-color microarray platforms, intensity-dependent lowess normalization is commonly used to correct relative gene expression values for biases. Here we outline a normalization method for use when the assumptions of lowess normalization fail. Specifically, this can occur when specialized boutique arrays are constructed that contain a subset of genes selected to test particular biological functions.

引用

页数：8

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