共 41 条
EB1 targets to kinetochores with attached, polymerizing microtubules
被引:139
作者:

Tirnauer, JS
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机构:
Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Canman, JC
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机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Salmon, ED
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机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Mitchison, TJ
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机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
机构:
[1] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
[2] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[3] Marine Biol Lab, Woods Hole, MA 02543 USA
关键词:
D O I:
10.1091/mbc.E02-04-0236
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Microtubule polymerization dynamics at kinetochores is coupled to chromosome movements, but its regulation there is poorly understood. The plus end tracking protein EB1 is required both for regulating microtubule dynamics and for maintaining a euploid genome. To address the role of EB1 in aneuploidy, we visualized its targeting in mitotic PtK1 cells. Fluorescent EB1, which localized to polymerizing ends of astral and spindle microtubules, was used to track their polymerization. EB1 also associated with a subset of attached kinetochores in late prometaphase and metaphase, and rarely in anaphase. Localization occurred in a narrow crescent, concanve toward the centromere, consistent with targeting to the microtubule plus end-kinetochore interface. EB1 did not localize to kinetochores lacking attached kinetochore microtubules in prophase or early prometaphase, or upon nocodazole treatment. By time lapse, EB1 specifically targeted to kinetochores moving antipoleward, coupled to microtubule plus end polymerization, and not during plus end depolymerization. It localized independently of spindle bipolarity, the spindle checkpoint, and dynein/dynactin function. EB1 is the first protein whose targeting reflects kinetochore directionality, unlike other plus end tracking proteins that show enhanced kinetochore binding in the absence of microtubules. Our results suggest EB1 may modulate kinetochore microtubule polymerization and/or attachment.
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页码:4308 / 4316
页数:9
相关论文
共 41 条
[1]
Regulation and function of the interaction between the APC tumour suppressor protein and EB1
[J].
Askham, JM
;
Moncur, P
;
Markham, AF
;
Morrison, EE
.
ONCOGENE,
2000, 19 (15)
:1950-1958

Askham, JM
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England

Moncur, P
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England

Markham, AF
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England

Morrison, EE
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England
[2]
Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form
[J].
Beinhauer, JD
;
Hagan, IM
;
Hegemann, JH
;
Fleig, U
.
JOURNAL OF CELL BIOLOGY,
1997, 139 (03)
:717-728

Beinhauer, JD
论文数: 0 引用数: 0
h-index: 0
机构: UNIV GIESSEN, INST MIKROBIOL & MOL BIOL, D-35392 GIESSEN, GERMANY

Hagan, IM
论文数: 0 引用数: 0
h-index: 0
机构: UNIV GIESSEN, INST MIKROBIOL & MOL BIOL, D-35392 GIESSEN, GERMANY

Hegemann, JH
论文数: 0 引用数: 0
h-index: 0
机构: UNIV GIESSEN, INST MIKROBIOL & MOL BIOL, D-35392 GIESSEN, GERMANY

Fleig, U
论文数: 0 引用数: 0
h-index: 0
机构: UNIV GIESSEN, INST MIKROBIOL & MOL BIOL, D-35392 GIESSEN, GERMANY
[3]
The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain
[J].
Berrueta, L
;
Tirnauer, JS
;
Schuyler, SC
;
Pellman, D
;
Bierer, BE
.
CURRENT BIOLOGY,
1999, 9 (08)
:425-428

论文数: 引用数:
h-index:
机构:

Tirnauer, JS
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

Schuyler, SC
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

Pellman, D
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

Bierer, BE
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA
[4]
The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules
[J].
Berrueta, L
;
Kraeft, SK
;
Tirnauer, JS
;
Schuyler, SC
;
Chen, LB
;
Hill, DE
;
Pellman, D
;
Bierer, BE
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1998, 95 (18)
:10596-10601

Berrueta, L
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Kraeft, SK
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Tirnauer, JS
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Schuyler, SC
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Chen, LB
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Hill, DE
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Pellman, D
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA

Bierer, BE
论文数: 0 引用数: 0
h-index: 0
机构: Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA
[5]
The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle
[J].
Canman, JC
;
Hoffman, DB
;
Salmon, ED
.
CURRENT BIOLOGY,
2000, 10 (10)
:611-614

Canman, JC
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

Hoffman, DB
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

Salmon, ED
论文数: 0 引用数: 0
h-index: 0
机构: Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[6]
LIS1, CLIP-170's key to the dynein/dynactin pathway
[J].
Coquelle, FM
;
Caspi, M
;
Cordelières, FP
;
Dompierre, JP
;
Dujardin, DL
;
Koifman, C
;
Martin, P
;
Hoogenraad, CC
;
Akhmanova, A
;
Galjart, N
;
De Mey, JR
;
Reiner, O
.
MOLECULAR AND CELLULAR BIOLOGY,
2002, 22 (09)
:3089-3102

Coquelle, FM
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Caspi, M
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Cordelières, FP
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Dompierre, JP
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Dujardin, DL
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Koifman, C
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Martin, P
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Hoogenraad, CC
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Akhmanova, A
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Galjart, N
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

De Mey, JR
论文数: 0 引用数: 0
h-index: 0
机构: Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

Reiner, O
论文数: 0 引用数: 0
h-index: 0
机构:
Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel
[7]
Evidence for a role of CLIP-170 in the establishment of metaphase chromosome alignment
[J].
Dujardin, D
;
Wacker, UI
;
Moreau, A
;
Schroer, TA
;
Rickard, JE
;
De Mey, JR
.
JOURNAL OF CELL BIOLOGY,
1998, 141 (04)
:849-862

Dujardin, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 06, Inst Jacques Monod, CNRS, Dept Supramol & Cellular Biol, F-75251 Paris 05, France

Wacker, UI
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 06, Inst Jacques Monod, CNRS, Dept Supramol & Cellular Biol, F-75251 Paris 05, France

Moreau, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 06, Inst Jacques Monod, CNRS, Dept Supramol & Cellular Biol, F-75251 Paris 05, France

Schroer, TA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 06, Inst Jacques Monod, CNRS, Dept Supramol & Cellular Biol, F-75251 Paris 05, France

Rickard, JE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 06, Inst Jacques Monod, CNRS, Dept Supramol & Cellular Biol, F-75251 Paris 05, France

De Mey, JR
论文数: 0 引用数: 0
h-index: 0
机构: Univ Paris 06, Inst Jacques Monod, CNRS, Dept Supramol & Cellular Biol, F-75251 Paris 05, France
[8]
Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis
[J].
Echeverri, CJ
;
Paschal, BM
;
Vaughan, KT
;
Vallee, RB
.
JOURNAL OF CELL BIOLOGY,
1996, 132 (04)
:617-633

Echeverri, CJ
论文数: 0 引用数: 0
h-index: 0
机构: WORCESTER FDN BIOMED RES, CELL BIOL GRP, SHREWSBURY, MA 01545 USA

Paschal, BM
论文数: 0 引用数: 0
h-index: 0
机构: WORCESTER FDN BIOMED RES, CELL BIOL GRP, SHREWSBURY, MA 01545 USA

Vaughan, KT
论文数: 0 引用数: 0
h-index: 0
机构: WORCESTER FDN BIOMED RES, CELL BIOL GRP, SHREWSBURY, MA 01545 USA

Vallee, RB
论文数: 0 引用数: 0
h-index: 0
机构: WORCESTER FDN BIOMED RES, CELL BIOL GRP, SHREWSBURY, MA 01545 USA
[9]
A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function
[J].
Faulkner, NE
;
Dujardin, DL
;
Tai, CY
;
Vaughan, KT
;
O'Connell, CB
;
Wangs, YL
;
Vallee, RB
.
NATURE CELL BIOLOGY,
2000, 2 (11)
:784-791

Faulkner, NE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA

Dujardin, DL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA

Tai, CY
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA

Vaughan, KT
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA

O'Connell, CB
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA

Wangs, YL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA

Vallee, RB
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01605 USA
[10]
Mutations in the APC tumour suppressor gene cause chromosomal instability
[J].
Fodde, R
;
Kuipers, J
;
Rosenberg, C
;
Smits, R
;
Kielman, M
;
Gaspar, C
;
van Es, JH
;
Bruekel, C
;
Wiegant, J
;
Giles, RH
;
Clevers, H
.
NATURE CELL BIOLOGY,
2001, 3 (04)
:433-438

论文数: 引用数:
h-index:
机构:

Kuipers, J
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Rosenberg, C
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Smits, R
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Kielman, M
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Gaspar, C
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

van Es, JH
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Bruekel, C
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Wiegant, J
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Giles, RH
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

Clevers, H
论文数: 0 引用数: 0
h-index: 0
机构: Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands