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Tri-iodothyronine upregulates adiponutrin mRNA expression in rat and human adipocytes

被引：14
作者：
Calvo, R. M. [1 ]
Obregon, M. J. [1 ]
机构：
[1] CSIC, Inst Invest Biomed Madrid, Madrid 28029, Spain
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2009年 / 311卷 / 1-2期
关键词：
Thyroid hormone; PNPLA3; Gene expression; Adipocytes; Rat epididymal adipose tissue; Human subcutaneous fat; WHITE ADIPOSE-TISSUE; PHOSPHOENOLPYRUVATE CARBOXYKINASE GTP; NONALCOHOLIC FATTY LIVER; HORMONE RESPONSE ELEMENT; LIPOPROTEIN-LIPASE GENE; THYROID-HORMONE; 3T3-L1; ADIPOCYTES; INSULIN; ENZYME; DIFFERENTIATION;
D O I：
10.1016/j.mce.2009.07.006
中图分类号：
Q2 [细胞生物学];
学科分类号：
071013 [干细胞生物学];
摘要：
Adiponutrin (PNPLA3) is expressed in adipose tissue. Although its precise function is unknown, some data suggest a dual role in lipid homeostasis. We have investigated the influence of thyroid hormone (TH) on PNPLA3 mRNA, in rat and human cultured white adipocytes and in rat white adipose tissue (WAT). Pnpla3 mRNA increased during differentiation of rat adipocytes in an insulin-dependent manner. Triiodothyronine further increased Pnpla3 expression at any day during differentiation and its effects were time and dose-dependent. The Pnpla3 mRNA half-life was stabilized by tri-iodothyronine, but a transcriptional component was also observed. Pnpla3 mRNA decreased in WAT of hypothyroid rats and was partially restored by treatment with TH. Taqman analysis showed that tri-iodothyronine also increased human PNPLA3 expression in cultured subcutaneous adipocytes from obese patients. In conclusion, PNPLA3 mRNA expression is upregulated by tri-iodothyronine in adipocytes in vitro. in humans and rats, and in vivo in rat WAT. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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页码：39 / 46
页数：8
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