Aluminum detoxification in Pseudomonas fluorescens is mediated by oxalate and phosphatidylethanolamine

被引:32
作者
Hamel, R [1 ]
Appanna, VD [1 ]
机构
[1] Laurentian Univ, Dept Chem & Biochem, Sudbury, ON P3E 2C6, Canada
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2003年 / 1619卷 / 01期
关键词
oxalate; phosphatidylethanolamine (PE); aluminum; isocitrate lyase;
D O I
10.1016/S0304-4165(02)00444-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-13 NMR studies with aluminum (Al)-stressed Pseudomonas fluorescens revealed that the trivalent metal was secreted in association with oxalate and phosphatidylethanolamine (PE). These moieties were observed in the insoluble pellet obtained upon incubation of these resting cells in the presence of either Al-citrate or citrate. This extrusion process was concomitant with the utilization of either of these tricarboxylic acids as a substrate. While only minimal amounts of At were secreted in the presence of such carbon source as glucose, succinate or oxaloacetate, oxalate did pen-nit the efflux of Al. Neither alpha-ketoglutarate nor ethylenediaminetetraacetic acid (EDTA) was effective in dislocating Al from the cells. The elimination of Al from the cells did not appear to be affected by p-dinitrophenol (DNP) or dicyclohexylcarbodiimide (DCCD) or azide, but was sensitive to temperature, pH and cerulenin, an inhibitor of lipid synthesis. Thus, it appears that R fluorescens detoxifies At via its extrusion in association with oxalate and PE in a process that apparently does not necessitate the direct utilization of energy. (C) 2002 Elsevier Science B.V All rights reserved.
引用
收藏
页码:70 / 76
页数:7
相关论文
共 29 条
[1]   The caveolae membrane system [J].
Anderson, RGW .
ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :199-225
[2]   A NOVEL ROLE FOR CALCITE IN CALCIUM HOMEOSTASIS [J].
ANDERSON, S ;
APPANNA, VD ;
HUANG, J ;
VISWANATHA, T .
FEBS LETTERS, 1992, 308 (01) :94-96
[3]  
APPANNA VD, 1994, FEMS MICROBIOL LETT, V124, P327
[4]  
APPANNA VD, IN PRESS CURR MICROB
[5]  
APPANNA VD, 1999, RRD MICROBIOL 2, V3, P615
[6]  
APPANNA VD, 1996, APPL ENVIRON MICROB, V62, P327
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   MOLECULAR-CLONING AND OVER-EXPRESSION OF THE GLYOXYLATE BYPASS OPERON FROM ESCHERICHIA-COLI ML308 [J].
ELMANSI, EMT ;
MACKINTOSH, C ;
DUNCAN, K ;
HOLMS, WH ;
NIMMO, HG .
BIOCHEMICAL JOURNAL, 1987, 242 (03) :661-665
[9]   QUANTITATIVE-DETERMINATION OF PHOSPHOLIPIDS USING THE DYES VICTORIA BLUE-R AND BLUE-B [J].
ERYOMIN, VA ;
POZNYAKOV, SP .
ANALYTICAL BIOCHEMISTRY, 1989, 180 (01) :186-191
[10]   THE CELLULAR TOXICITY OF ALUMINUM [J].
EXLEY, C ;
BIRCHALL, JD .
JOURNAL OF THEORETICAL BIOLOGY, 1992, 159 (01) :83-98