Panton-Valentine leukocidin genes in a phage-like particle isolated from mitomycin C-treated Staphylococcus aureus V8 (ATCC 49775)

被引：70

作者：

Kaneko, J

Kimura, T

Kawakami, Y

Tomita, T

Kamio, Y

机构：

[1] TOHOKU UNIV, FAC AGR, DEPT APPL BIOL CHEM, AOBA KU, SENDAI, MIYAGI 981, JAPAN

[2] SHINSHU UNIV, SCH ALLIED MED SCI, DEPT MED TECHNOL, DIV CLIN MICROBIOL, MATSUMOTO, NAGANO 390, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 11期

关键词：

Staphylococcus aureus V8 (ATCC 49775); lukS-PV; LukF-PV; integrase; temperate phage (phi PVL) carrying Panton-Valentine leukocidin genes;

D O I：

10.1271/bbb.61.1960

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The staphylococcal Panton-Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (phi PVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in phi PVL was a linear double-stranded 40-kh DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase gene) of phi PVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.

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页码：1960 / 1962

页数：3

相关论文

共 15 条

[1] THE INTEGRASE FAMILY OF SITE-SPECIFIC RECOMBINASES - REGIONAL SIMILARITIES AND GLOBAL DIVERSITY [J].

ARGOS, P ;

LANDY, A ;

ABREMSKI, K ;

EGAN, JB ;

HAGGARDLJUNGQUIST, E ;

HOESS, RH ;

KAHN, ML ;

KALIONIS, B ;

NARAYANA, SVL ;

PIERSON, LS ;

STERNBERG, N ;

LEONG, JM .

EMBO JOURNAL, 1986, 5 (02) :433-440

[2] NOVEL ORGANIZATION OF THE SITE-SPECIFIC INTEGRATION AND EXCISION RECOMBINATION FUNCTIONS OF THE STAPHYLOCOCCUS-AUREUS SEROTYPE-F VIRULENCE-CONVERTING PHAGES PHI-13 AND PHI-42 [J].

CARROLL, D ;

KEHOE, MA ;

CAVANAGH, D ;

COLEMAN, DC .

MOLECULAR MICROBIOLOGY, 1995, 16 (05) :877-893

[3] GENETIC ORGANIZATION AND SEQUENCE OF THE REGION ENCODING INTEGRATIVE FUNCTIONS FROM LACTOBACILLUS-GASSERI TEMPERATE BACTERIOPHAGE-PHI ADH [J].

FREMAUX, C ;

DEANTONI, GL ;

RAYA, RR ;

KLAENHAMMER, TR .

GENE, 1993, 126 (01) :61-66

[4] Gene of LukF-PV-like component of Panton-Valentine leukocidin in Staphylococcus aureus P83 is linked with lukM [J].

Kaneko, J ;

Muramoto, K ;

Kamio, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (03) :541-544

[5] THE TOXIC SHOCK SYNDROME EXOTOXIN STRUCTURAL GENE IS NOT DETECTABLY TRANSMITTED BY A PROPHAGE [J].

KREISWIRTH, BN ;

LOFDAHL, S ;

BETLEY, MJ ;

OREILLY, M ;

SCHLIEVERT, PM ;

BERGDOLL, MS ;

NOVICK, RP .

NATURE, 1983, 305 (5936) :709-712

[6] INTEGRATION OF STAPHYLOCOCCAL PHAGE L54A OCCURS BY SITE-SPECIFIC RECOMBINATION - STRUCTURAL-ANALYSIS OF THE ATTACHMENT SITES [J].

LEE, CY ;

IANDOLO, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (15) :5474-5478

[7] STRUCTURAL-ANALYSIS OF STAPHYLOCOCCAL BACTERIOPHAGE PHI-11 ATTACHMENT SITES [J].

LEE, CY ;

IANDOLO, JJ .

JOURNAL OF BACTERIOLOGY, 1988, 170 (05) :2409-2411

[8] CHARACTERIZATION OF GENETIC ELEMENTS REQUIRED FOR SITE-SPECIFIC INTEGRATION OF THE TEMPERATE LACTOCOCCAL BACTERIOPHAGE PHI-LC3 AND CONSTRUCTION OF INTEGRATION-NEGATIVE PHI-LC3 MUTANTS [J].

LILLEHAUG, D ;

BIRKELAND, NK .

JOURNAL OF BACTERIOLOGY, 1993, 175 (06) :1745-1755

[9]

Maniatis T., 1982, MOL CLONING

[10] Bacteriophage T12 of Streptococcus pyogenes integrates into the gene encoding a serine tRNA [J].

Mcshan, WM ;

Tang, YF ;

Ferretti, JJ .

MOLECULAR MICROBIOLOGY, 1997, 23 (04) :719-728