Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin

被引：304

作者：

Lu, Qing ^{[1
,2
]}

Tang, Xurong ^{[1
,3
]}

Tian, Gang ^{[1
,4
]}

Wang, Fang ^{[1
,5
]}

Liu, Kede ^{[1
,5
]}

Nguyen, Vi ^{[1
]}

Kohalmi, Susanne E. ^{[4
]}

Keller, Wilfred A. ^{[3
]}

Tsang, Edward W. T. ^{[3
]}

Harada, John J. ^{[6
]}

Rothstein, Steven J. ^{[2
]}

Cui, Yuhai ^{[1
]}

机构：

[1] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada

[2] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada

[3] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

[4] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada

[5] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

[6] Univ Calif Davis, Plant Biol Sect, Coll Biol Sci, Davis, CA 95616 USA

来源：

PLANT JOURNAL | 2010年 / 61卷 / 02期

关键词：

TREX-2 mRNA export complex; nucleoporin NUP1; THP1; SAC3; DSS1; NUCLEAR-PORE COMPLEX; FUNCTIONAL COMPONENT; STRESS RESPONSES; FISSION YEAST; PROTEASOME; TRANSCRIPTION; PROTEIN; GENES; SAGA; DSS1;

D O I：

10.1111/j.1365-313X.2009.04048.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>Nuclear pore complexes (NPCs) are vital to nuclear-cytoplasmic communication in eukaryotes. The yeast NPC-associated TREX-2 complex, also known as the Thp1-Sac3-Cdc31-Sus1 complex, is anchored on the NPC via the nucleoporin Nup1, and is essential for mRNA export. Here we report the identification and characterization of the putative Arabidopsis thaliana TREX-2 complex and its anchoring nucleoporin. Physical and functional evidence support the identification of the Arabidopsis orthologs of yeast Thp1 and Nup1. Of three Arabidopsis homologs of yeast Sac3, two are putative TREX-2 components, but, surprisingly, none are required for mRNA export as they are in yeast. Physical association of the two Cdc31 homologs, but not the Sus1 homolog, with the TREX-2 complex was observed. In addition to identification of these TREX-2 components, direct interactions of the Arabidopsis homolog of DSS1, which is an established proteasome component in yeast and animals, with both the TREX-2 complex and the proteasome were observed. This suggests the possibility of a link between the two complexes. Thus this work has identified the putative Arabidopsis TREX-2 complex and provides a foundation for future studies of nuclear export in Arabidopsis.

引用

页码：259 / 270

页数：12

共 52 条

[1] Regulation and epigenetic control of transcription at the nuclear periphery [J].

Ahmed, Sara ;

Brickner, Jason H. .

TRENDS IN GENETICS, 2007, 23 (08) :396-402

[2] The nuclear envelope and transcriptional control [J].

Akhtar, Asifa ;

Gasser, Susan M. .

NATURE REVIEWS GENETICS, 2007, 8 (07) :507-517

[3] Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II [J].

Baillat, D ;

Hakimi, MA ;

Näär, AM ;

Shilatifard, A ;

Cooch, N ;

Shiekhattar, R .

CELL, 2005, 123 (02) :265-276

[4] GENE GATING - A HYPOTHESIS [J].

BLOBEL, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (24) :8527-8529

[5] SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope [J].

Cabal, Ghislain G. ;

Genovesio, Auguste ;

Rodriguez-Navarro, Susana ;

Zimmer, Christophe ;

Gadal, Olivier ;

Lesne, Annick ;

Buc, Henri ;

Feuerbach-Fournier, Frank ;

Olivo-Marin, Jean-Christophe ;

Hurt, Eduard C. ;

Nehrbass, Ulf .

NATURE, 2006, 441 (7094) :770-773

[6]

Chinnusamy V, 2008, CURR TOP MICROBIOL, V326, P235

[7] The eer5 mutation, which affects a novel proteasome-related subunit, indicates a prominent role for the COP9 signalosome in resetting the ethylene-signaling pathway in Arabidopsis [J].

Christians, Matthew J. ;

Robles, Linda M. ;

Zeller, Samantha M. ;

Larsen, Paul B. .

PLANT JOURNAL, 2008, 55 (03) :467-477

[8] The PAM domain, a multi-protein complex-associated module with an all-alpha-helix fold [J].

Ciccarelli, FD ;

Izaurralde, E ;

Bork, P .

BMC BIOINFORMATICS, 2003, 4 (1)

[9] Transport of messenger RNA from the nucleus to the cytoplasm [J].

Cole, Charles N. ;

Scarcelli, John J. .

CURRENT OPINION IN CELL BIOLOGY, 2006, 18 (03) :299-306

[10] Structure, dynamics and function of nuclear pore complexes [J].

D'Angelo, Maximiliano A. ;

Hetzer, Martin W. .

TRENDS IN CELL BIOLOGY, 2008, 18 (10) :456-466

← 1 2 3 4 5 6 →