A comparative electrophoretic analysis of mammalian hair and avian feather proteins

被引：6

作者：

Khawar, SL ^{[1
]}

Watson, K ^{[1
]}

Jones, GL ^{[1
]}

机构：

[1] UNIV NEW ENGLAND,DEPT CELLULAR & MOL BIOL,ARMIDALE,NSW 2351,AUSTRALIA

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 1997年 / 29卷 / 02期

关键词：

feather proteins; hair proteins; laser densitometry; radioactive labelling; SDS electrophoresis;

D O I：

10.1016/S1357-2725(96)00081-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved one-dimensional sodium dodecyl sulphate separation has allowed a detailed electrophoretic comparison of the proteins that constitute hairs and feathers isolated from various representative samples of mammalian, both placental and marsupial and avian species. The proteins from various species were S-carboxymethylated using labelled (C-14)-iodoacetic acid and detected both by Coomassie stain and fluorography. Proteins from mammalian species were compared with respect to their molecular masses and relative cysteine content using laser densitometry of both Coomassie stained gels and fluorographs of C-14 labelled gels. These results may be quite useful forensically as an adjunct to fibre analysis. (C) 1997 Published by Elsevier Science Ltd.

引用

页码：367 / 380

页数：14

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