Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis

被引：35

作者：

Hladnik, U

Braida, L

Boniotto, M

Pirulli, D

Gerin, F

Amoroso, A

Crovella, S

机构：

[1] Univ Trieste, Sez Genet, Dipartimento Sci Riprod & Sviluppo, I-34100 Trieste, Italy

[2] IRCCS Burlo Garofolo, Serv Genet, I-34100 Trieste, Italy

来源：

CLINICAL AND EXPERIMENTAL MEDICINE | 2002年 / 2卷 / 02期

关键词：

MBL-2; polymorphisms; melting temperature analysis; automatic allele calling;

D O I：

10.1007/s102380200014

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.

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页码：105 / 108

页数：4

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