Cellular catabolism in apoptosis: DNA degradation and endonuclease activation

被引：46

作者：

Montague, JW ^{[1
]}

Cidlowski, JA ^{[1
]}

机构：

[1] NIEHS, RES TRIANGLE PK, NC 27709 USA

来源：

EXPERIENTIA | 1996年 / 52卷 / 10-11期

关键词：

apoptosis; cyclophilin; DNA degradation; NUC18; endonuclease;

D O I：

10.1007/BF01920104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recent research has focused on identifying the biochemical events associated with the apoptotic process. These include specific degradation of the chromatin which was described by Wyllie in 1980 [1], with the report of the appearance of discretely sized DNA fragments from apoptotic rat thymocytes. The fragments corresponded in size to strands of DNA that were cleaved at internucleosomal regions and create a 'ladder pattern' when electrophoresed on an agarose gel. Because of its near universality, internucleosomal DNA degradation is considered a diagnostic hallmark of cells undergoing apoptosis. It is of great interest to identify the enzymes involved, and some of the candidates will be discussed.

引用

页码：957 / 962

页数：6

共 56 条

[1]

ARENDS MJ, 1990, AM J PATHOL, V136, P593

[2]

ARENDS MJ, 1991, INT REV EXP PATHOL, V32, P223

[3] IDENTIFICATION OF DEOXYRIBONUCLEASE-II AS AN ENDONUCLEASE INVOLVED IN APOPTOSIS [J].