Hyaluronan synthase of chlorella virus PBCV-1

被引：104

作者：

DeAngelis, PL ^{[1
]}

Jing, W ^{[1
]}

Graves, MV ^{[1
]}

Burbank, DE ^{[1
]}

VanEtten, JL ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT PLANT PATHOL,LINCOLN,NE 68583

来源：

SCIENCE | 1997年 / 278卷 / 5344期

关键词：

D O I：

10.1126/science.278.5344.1800

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sequence analysis of the 330-kilobase genome of the virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases. Hyaluronan is an essential polysaccharide found in higher animals as well as in a few pathogenic bacteria. Expression of the A98R gene product in Escherichia coli indicated that the recombinant protein is an authentic hyaluronan synthase. A98R is expressed early in PBCV-1 infection and hyaluronan is produced in infected algae. These results demonstrate that a virus can encode an enzyme capable of synthesizing a carbohydrate polymer and that hyaluronan exists outside of animals and their pathogens.

引用

页码：1800 / 1803

页数：4

共 34 条

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