Characterization and functional analysis of the promoter for the human equilibrative nucleoside transporter gene, hENT1

被引：20

作者：

Abdulla, Parween ^{[1
]}

Coe, Imogen R. ^{[1
]}

机构：

[1] York Univ, Dept Biol, N York, ON M3J 1P3, Canada

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2007年 / 26卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

hENT1; gene; promoter;

D O I：

10.1080/15257770601052331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Equilibrative nucleoside transporters (ENTs) are membrane proteins that transport nucleosides, nucleobases and analogs across membranes. ENT genes and the regulation of their expression are poorly understood. Therefore, we isolated and functionally characterized the promoter of the prototypic human ENT, hENT1. A single transcriptional initiation site 58 bp downstream of the TATA box and 272 bp upstream of the translation initiation site is present. Limited sequence similarity exists between the hENT1 and mouse ENT1 (mENT1) promoters suggesting conservation of ENT1 transcriptional regulators in mammals. Putative consensus sites for transcription factors exist within the hENT1 promoter. Reporter assays revealed similar but not identical transcriptional activity profiles in human cells.

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收藏

页码：99 / 110

页数：12

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