SKPs Derive from Hair Follicle Precursors and Exhibit Properties of Adult Dermal Stem Cells

被引:283
作者
Biernaskie, Jeffrey [5 ]
Paris, Maryline [5 ]
Morozova, Olena [3 ]
Fagan, B. Matthew [4 ]
Marra, Marco [3 ]
Pevny, Larysa [4 ]
Miller, Freda D. [1 ,2 ,5 ]
机构
[1] Univ Toronto, Dept Mol Genet, Toronto, ON M5G 1X5, Canada
[2] Univ Toronto, Dept Physiol, Toronto, ON M5G 1X5, Canada
[3] BC Canc Agcy, Genome Sci Ctr, Vancouver, BC V5Z 4S6, Canada
[4] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA
[5] Univ Toronto, Hosp Sick Children, Dev & Stem Cell Biol Grp, Toronto, ON M5G 1X5, Canada
关键词
SKIN; GROWTH; GENERATION; MOUSE; RAT; DIFFERENTIATE; IMPLANTATION; ASTROCYTES; CONTRIBUTE; INDUCTION;
D O I
10.1016/j.stem.2009.10.019
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Despite the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we investigated whether skin-derived precursors (SKPs) might fulfill such a role. We show that SKPs derive from Sox2(+) hair follicle dermal cells and that these two cell populations are similar with regard to their transcriptome and functional properties. Both clonal SKPs and endogenous Sox2(+) cells induce hair morphogenesis, differentiate into dermal cell types, and home to a hair follicle niche upon transplantation. Moreover, hair follicle-derived SKPs; self-renew, maintain their multipotency, and serially reconstitute hair follicles. Finally, grafting experiments show that follicle-associated dermal cells move out of their niche to contribute cells for dermal maintenance and wound-healing. Thus, SKPs derive from Sox2(+) follicle-associated dermal precursors and display functional properties predicted of a dermal stem cell, contributing to dermal maintenance, wound-healing, and hair follicle morphogenesis.
引用
收藏
页码:610 / 623
页数:14
相关论文
共 27 条
[1]   Multipotent cell lineages in early mouse development depend on SOX2 function [J].
Avilion, AA ;
Nicolis, SK ;
Pevny, LH ;
Perez, L ;
Vivian, N ;
Lovell-Badge, R .
GENES & DEVELOPMENT, 2003, 17 (01) :126-140
[2]   Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny [J].
Biernaskie, Jeffrey A. ;
McKenzie, Ian A. ;
Toma, Jean G. ;
Miller, Freda D. .
NATURE PROTOCOLS, 2006, 1 (06) :2803-2812
[3]   Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis [J].
Driskell, Ryan R. ;
Giangreco, Adam ;
Jensen, Kim B. ;
Mulder, Klaas W. ;
Watt, Fiona M. .
DEVELOPMENT, 2009, 136 (16) :2815-2823
[4]   SOX2, a persistent marker for multipotential neural stem cells derived from embryonic stem cells, the embryo or the adult [J].
Ellis, P ;
Fagan, BM ;
Magness, ST ;
Hutton, S ;
Taranova, O ;
Hayashi, S ;
McMahon, A ;
Rao, M ;
Pevny, L .
DEVELOPMENTAL NEUROSCIENCE, 2004, 26 (2-4) :148-165
[5]   Analysis of the neurogenic potential of multipotent skin-derived precursors [J].
Fernandes, Karl J. L. ;
Kobayashi, Nao R. ;
Gallagher, Conor J. ;
Barnabe-Heider, Fanie ;
Aumont, Anne ;
Kaplan, David R. ;
Miller, Freda D. .
EXPERIMENTAL NEUROLOGY, 2006, 201 (01) :32-48
[6]   A dermal niche for multipotent adult skin-derived precursor cells [J].
Fernandes, KJL ;
McKenzie, IA ;
Mill, P ;
Smith, KM ;
Akhavan, M ;
Barnabé-Heider, F ;
Biernaskie, J ;
Junek, A ;
Kobayashi, NR ;
Toma, JG ;
Kaplan, DR ;
Labosky, PA ;
Rafuse, V ;
Hui, CC ;
Miller, FD .
NATURE CELL BIOLOGY, 2004, 6 (11) :1082-U16
[7]  
FUCHS E, 2003, EXP DERMATOL, V12, P126
[8]   Generating green fluorescent mice by germline transmission of green fluorescent ES cells [J].
Hadjantonakis, AK ;
Gertsenstein, M ;
Ikawa, M ;
Okabe, M ;
Nagy, A .
MECHANISMS OF DEVELOPMENT, 1998, 76 (1-2) :79-90
[9]   A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin [J].
Hunt, David P. J. ;
Morris, Paul N. ;
Sterling, Jane ;
Anderson, Jane A. ;
Joannides, Alexis ;
Jahoda, Colin ;
Compston, Alastair ;
Chandran, Siddharthan .
STEM CELLS, 2008, 26 (01) :163-172
[10]  
IBRAHIM L, 1982, J EMBRYOL EXP MORPH, V72, P209