25-hydroxyvitamin D-3 1 alpha-hydroxylase and vitamin D synthesis

被引：383

作者：

Takeyama, K

Kitanaka, S

Sato, T

Kobori, M

Yanagisawa, J

Kato, S

机构：

[1] UNIV TOKYO, INST MOL & CELLULAR BIOSCI, BUNKYO KU, TOKYO 113, JAPAN

[2] JAPAN SCI & TECHNOL, CREST, KAWAGUCHI, SAITAMA 332, JAPAN

[3] YAMANOUCHI PHARMACEUT CO LTD, INST DRUG DISCOVERY RES, MOL MED LABS, TSUKUBA, IBARAKI 305, JAPAN

来源：

SCIENCE | 1997年 / 277卷 / 5333期

关键词：

D O I：

10.1126/science.277.5333.1827

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Renal 25-hydroxyvitamin D-3 1 alpha-hydroxylase [1 alpha(OH)ase] catalyzes metabolic activation of 25-hydroxyvitamin D-3 into 1 alpha,25-dihydroxyvitamin D-3 [1 alpha,25(OH)(2)D-3], an active form of vitamin D, and is inhibited by 1 alpha,25(OH)(2)D-3. 1 alpha(OH)ase, which was cloned from the kidney of mice lacking the vitamin D receptor (VDR-/- mice), is a member of the P450 family of enzymes (P450(VD1 alpha)). Expression of 1 alpha(OH)ase was suppressed by 1 alpha,25(OH)(2)D-3 in VDR+/+ and VDR+/- mice but not in VDR-/- mice. These results indicate that the negative feedback regulation of active vitamin D synthesis is mediated by 1 alpha(OH)ase through liganded VDR.

引用

页码：1827 / 1830

页数：4

共 55 条

[1] ISOLATION AND STRUCTURAL IDENTIFICATION OF 1,25-DIHYDROXYVITAMIN-D3 PRODUCED BY CULTURED ALVEOLAR MACROPHAGES IN SARCOIDOSIS [J].