Identification, sequence analysis and expression of transcripts encoding a putative metalloproteinase, eMDC II, in human and macaque epididymis

被引：16

作者：

Jury, JA ^{[1
]}

Perry, ACF ^{[1
]}

Hall, L ^{[1
]}

机构：

[1] Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England

来源：

MOLECULAR HUMAN REPRODUCTION | 1999年 / 5卷 / 12期

关键词：

cysteine-rich protein; disintegrin-like; epididymis; metalloproteinase; metalloproteinase-like;

D O I：

10.1093/molehr/5.12.1127

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The metalloproteinase-like, disintegrin-like, cysteine-rich (MDC) family is a large group of sequence-related proteins, first characterized in the male reproductive tract, but subsequently also identified in non-reproductive tissues. Their primary translation products are of similar to 90 kDa and each can be divided into distinct domains which show remarkable homology to reprolysins; snake venom haemorrhagic components possessing metalloproteinase and/or disintegrin domains, Several MDC proteins are abundantly-expressed in the male reproductive tract, suggesting functions in fertility. We now describe the cloning, sequence determination and characterization of transcripts encoding the human and macaque (Macaca fascicularis) orthologues of a novel member of the MDC family (eMDC II) which is abundantly-expressed in the epididymis. Unlike many MDC proteins expressed in the reproductive tract, eMDC II possesses the extended 'catalytic centre' consensus sequence characteristic of a reprolysin-like metalloproteinase. This suggests that eMDC II has proteolytic activity.

引用

页码：1127 / 1134

页数：8

共 38 条

[1] MOUSE EGG INTEGRIN ALPHA-6-BETA-1 FUNCTIONS AS A SPERM RECEPTOR [J].

ALMEIDA, EAC ;

HUOVILA, APJ ;

SUTHERLAND, AE ;

STEPHENS, LE ;

CALARCO, PG ;

SHAW, LM ;

MERCURIO, AM ;

SONNENBERG, A ;

PRIMAKOFF, P ;

MYLES, DG ;

WHITE, JM .

CELL, 1995, 81 (07) :1095-1104

[2] SEQUENCE AND EXPRESSION OF A MONKEY TESTICULAR TRANSCRIPT ENCODING TMDC-I, A NOVEL MEMBER OF THE METALLOPROTEINASE-LIKE, DISINTEGRIN-LIKE, CYSTEINE-RICH (MDC) PROTEIN FAMILY [J].

BARKER, HL ;

PERRY, ACF ;

JONES, R ;

HALL, L .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1218 (03) :429-431

[3] A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells [J].

Black, RA ;

Rauch, CT ;

Kozlosky, CJ ;

Peschon, JJ ;

Slack, JL ;

Wolfson, MF ;

Castner, BJ ;

Stocking, KL ;

Reddy, P ;

Srinivasan, S ;

Nelson, N ;

Boiani, N ;

Schooley, KA ;

Gerhart, M ;

Davis, R ;

Fitzner, JN ;

Johnson, RS ;

Paxton, RJ ;

March, CJ ;

Cerretti, DP .

NATURE, 1997, 385 (6618) :729-733

[4]

Cho CH, 1997, DEV GENET, V20, P320, DOI 10.1002/(SICI)1520-6408(1997)20:4&lt

[5]

320::AID-DVG3&gt

[6]

3.0.CO

[7]

2-9

[8] ADAM7, a member of the ADAM (a disintegrin and metalloprotease) gene family is specifically expressed in the mouse anterior pituitary and epididymis [J].

Cornwall, GA ;

Hsia, N .

ENDOCRINOLOGY, 1997, 138 (10) :4262-4272

[9] INTERACTIONS BETWEEN RAT EPIDIDYMAL EPITHELIUM AND SPERMATOZOA [J].

FORNES, MW ;

DEROSAS, JC .

ANATOMICAL RECORD, 1991, 231 (02) :193-200

[10] The gene for the human tMDC I sperm surface protein is non-functional: implications for its proposed role in mammalian sperm-egg recognition [J].

Frayne, J ;

Hall, L .

BIOCHEMICAL JOURNAL, 1998, 334 :171-176

← 1 2 3 4 →