Molecularly imprinted polymer for solid-phase extraction of ephedrine and analogs from human plasma

被引:32
作者
Lasakova, Martina [1 ,2 ]
Thiebaut, Didier [1 ]
Jandera, Pavel [2 ]
Pichon, Valerie [1 ]
机构
[1] ESPCI ParisTech, UMR PECSA 7195, Dept Analyt & Environm Chem, CNRS, F-75231 Paris 05, France
[2] Univ Pardubice, Dept Analyt Chem, Pardubice, Czech Republic
关键词
Ephedrine; Human plasma; Molecularly imprinted polymer; SPE; SELECTIVE EXTRACTION; SAMPLE PREPARATION; RECOGNITION; PERFORMANCE; SEPARATION; TRIAZINES; CLEANUP; ONLINE; URINE; MISPE;
D O I
10.1002/jssc.200800684
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A molecularly imprinted polymer (MIP) was synthesized and evaluated to selectively extract ephedrine from human plasma. The MIP synthesis was performed in chloroform with methacrylic acid as a functional monomer and the target alkaloid as a template molecule. The resulting MIP was applied to the selective extraction of ephedrine from a pure aqueous medium. A recovery about 74% was obtained using the MIP with only 7% on the nonimprinted polymer (NIP). A very straightforward selective SPE procedure was then successfully applied to the direct extraction of ephedrine from spiked human plasma with a high extraction recovery (68%) on the MIP with no recovery on the NIP. Moreover, the MIP was used for the selective extraction of catecholamine neurotransmitters, i.e. adrenaline and noradrenaline.
引用
收藏
页码:1036 / 1042
页数:7
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